AB126628

重组Anti-PDLIM1/CLP36抗体[EPR7187]

Anti-PDLIM1/CLP36 antibody [EPR7187]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal PDLIM1/CLP36 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

CLIM1, CLP36, PDLIM1, PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (AB126628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (AB126628)

ab126628 at 1/250 staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.

Unknown

Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (AB126628)

All lanes:

Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Caco 2 cell lysate at 10 µg

Lane 5:

Saos 2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 36 kDa

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CiteAb

Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (AB126628)

PDLIM1/CLP36 western blot using anti-PDLIM1/CLP36 antibody [EPR7187] ab126628. Publication image and figure legend from Hayashi, G. & Cortopassi, G., 2016, PLoS One, PubMed 27078885.

ab126628 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126628 please see the product overview.

Tertiary screening of potential Friedreich's ataxia protein level biomarkers by western blot.The target western blot fluorescence signals were normalized to β-Tubulin. The frataxin expression in the FA cell was 0.15 fold of the healthy control b-lymphoblasts. On the protein level, four of the six biomarkers were consistent with the secondary qPCR screening : PDLIM1 (1.35 fold), NCF2 (0.36 fold), PRDX5 (0.44 fold) and SFTPD (0.47 fold). Bars represent averages±standard deviations (n = 4, p<0.05*, p<0.01**, p<0.001***). Data in S3 File.

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不同偶联物与剂型 (1)

Carrier free

Anti-PDLIM1/CLP36 antibody [EPR7187] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7187

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM1 also known as CLP36 is a protein that plays a mechanical role as an actin-binding protein. It is part of the PDZ-LIM family of proteins characterized by the presence of PDZ and LIM domains. PDLIM1 has a molecular mass of approximately 36 kDa and is involved in cytoskeletal organization by connecting actin filaments to the cross-linked network. It expresses mostly in tissues like the heart skeletal muscles and to some degree in others such as liver and lungs.

Biological function summary

PDLIM1 connects to actin filaments helping in maintaining cytoskeletal dynamics and cell structure. This protein is part of the larger complex involved in organizing actin filaments. PDLIM1 through interactions with cytoskeletal components regulates muscle contraction and cell motility. It stabilizes actin filaments through its PDZ domain and modulates signal transduction pathways linked to cellular adhesion and movement.

Pathways

Studies have identified PDLIM1's involvement in the regulation of signaling pathways such as the MAPK and Wnt pathways. Through these processes PDLIM1 interacts with proteins like kinases and phosphatases which play a role in signal transduction and cellular communication. By modulating these pathways PDLIM1 influences cell growth and differentiation processes critical in tissue development and maintenance.

PDLIM1 is linked to conditions involving muscle malfunction and some cancer forms. Abnormal expression of PDLIM1 correlates with cardiomyopathies as it affects heart muscle function. Also changes in PDLIM1 levels have connections to several cancers possibly through pathways involving proteins such as β-catenin an important player in cell proliferation and tumor growth. Understanding these links may provide insights into novel therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed : 10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

See full target information PDLIM1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0153574 PubMed27078885

2016

Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia.

Applications

Species

Unspecified reactive species

Genki Hayashi,Gino Cortopassi

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PDLIM1/CLP36抗体[EPR7187]