AB129015

重组Anti-PDLIM1/CLP36抗体[EPR7186(B)]

Anti-PDLIM1/CLP36 antibody [EPR7186(B)]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal PDLIM1/CLP36 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

CLIM1, CLP36, PDLIM1, PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36

4 Images

Flow Cytometry (Intracellular) - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by intracellular flow cytometry or a Rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

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Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

All lanes:

Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution

Lane 1:

Human heart cell lysate at 10 µg

Lane 2:

L6 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Caco 2 cell lysate at 10 µg

Lane 5:

Saos 2 cell lysate at 10 µg

Lane 6:

C2C12 cell lysate at 10 µg

Lane 7:

Rat lung cell lysate at 10 µg

Lane 8:

Mouse lung cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 36 kDa

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OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

OI-RD Scanning

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OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PDLIM1/CLP36 antibody [EPR7186(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7186(B)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM1 also known as CLP36 is a protein that plays a mechanical role as an actin-binding protein. It is part of the PDZ-LIM family of proteins characterized by the presence of PDZ and LIM domains. PDLIM1 has a molecular mass of approximately 36 kDa and is involved in cytoskeletal organization by connecting actin filaments to the cross-linked network. It expresses mostly in tissues like the heart skeletal muscles and to some degree in others such as liver and lungs.

Biological function summary

PDLIM1 connects to actin filaments helping in maintaining cytoskeletal dynamics and cell structure. This protein is part of the larger complex involved in organizing actin filaments. PDLIM1 through interactions with cytoskeletal components regulates muscle contraction and cell motility. It stabilizes actin filaments through its PDZ domain and modulates signal transduction pathways linked to cellular adhesion and movement.

Pathways

Studies have identified PDLIM1's involvement in the regulation of signaling pathways such as the MAPK and Wnt pathways. Through these processes PDLIM1 interacts with proteins like kinases and phosphatases which play a role in signal transduction and cellular communication. By modulating these pathways PDLIM1 influences cell growth and differentiation processes critical in tissue development and maintenance.

PDLIM1 is linked to conditions involving muscle malfunction and some cancer forms. Abnormal expression of PDLIM1 correlates with cardiomyopathies as it affects heart muscle function. Also changes in PDLIM1 levels have connections to several cancers possibly through pathways involving proteins such as β-catenin an important player in cell proliferation and tumor growth. Understanding these links may provide insights into novel therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed : 10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

See full target information PDLIM1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:5820 PubMed37037887

2023

PDLIM1 inhibits cell migration and invasion in diabetic retinopathy via negatively regulating Wnt3a.

Applications

Unspecified application

Species

Unspecified reactive species

Pinxue Xie,Qisheng You,Jiang Zhu,Wuxiang Xie,Ping Wei,Siquan Zhu,Yunhui Du,Xinxiao Gao

Journal of oncology 2022:5456016 PubMed36164345

2022

miR-187/PDLIM1 Gets Involved in Gastric Cancer Progression and Cisplatin Sensitivity of Cisplatin by Mediating the Hippo-YAP Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yeru Tan,Yuehua Li,Hongbo Zhu,Xiaoping Wu,Kai Mei,Pian Li,Qiao Yang

Theranostics 12:5051-5068 PubMed35836803

2022

CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Lu,Yongxin Mu,Ju Chen,Xinyuan Guan,Ling Guo,Chuanyue Wu

Scientific reports 5:18327 PubMed26679095

2015

PDLIM1 inhibits NF-κB-mediated inflammatory signaling by sequestering the p65 subunit of NF-κB in the cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Rumiko Ono,Tsuneyasu Kaisho,Takashi Tanaka

View all publications

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Abcam Product Promise

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重组Anti-PDLIM1/CLP36抗体[EPR7186(B)]