Anti-PDK1抗体[4A11]
Anti-PDK1 antibody [4A11]
4
(2 Reviews)
|
(51 Publications)
Mouse Monoclonal PDK1 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Human, Mouse, African green monkey, Rat, Monkey samples. Cited in 51 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDK1 aa 300 to C-terminus.
查看别名
PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [4A11] (AB110025)
Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [4A11] (AB110025)
Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow Cyt
Unknown
Flow Cytometry - Anti-PDK1 antibody [4A11] (AB110025)
Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [4A11] (AB110025)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
- WB
Supplier Data
Western blot - Anti-PDK1 antibody [4A11] (AB110025)
12% SDS gel.
Block the membrane for 1 h at room temperature or overnight at 4°C using blocking buffer, 5% milk.
Incubate in ab110025 overnight at 4°C.
Incubation at room temperature for 1 hour with the secondary antibody.
Lanes 1 - 2:
Western blot - Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 3:
Beta-actin antibody at 1/10000 dilution
Lane 1:
Jurkat cell lysate at 15 µL
Lane 2:
HeLa cell lysate at 15 µL
Lane 3:
NIH3T3 cell lysate at 15 µL
Secondary
All lanes:
HRP-Goat Anti-Mouse IgG (Fc) at 1/10000 dilution
Predicted band size: 49 kDa
false
- WB
Unknown
Western blot - Anti-PDK1 antibody [4A11] (AB110025)
All lanes:
Western blot - Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 1:
NIH3T3 cell lysate
Lane 2:
Hela cell lysate
Lane 3:
Jurkat cell lysate
Lane 4:
HepG2 cell lysate
Lane 5:
PC-12 cell lysate
Lane 6:
Cos7 cell lysate
Predicted band size: 49 kDa
false
反应性数据
性能和储存信息
形式
纯化工艺
纯化说明
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
分装信息
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产品实验方案
- Visit the General protocols
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靶点信息
文献 (51)
Recent publications for all applications. Explore the full list and refine your search
BMC biology 22:302 PubMed39736678
2024
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Acta biochimica et biophysica Sinica 57:712-726 PubMed39578715
2024
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Cells 13: PubMed39404433
2024
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Cell death & disease 15:657 PubMed39242557
2024
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Molecular medicine reports 30: PubMed39219283
2024
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Redox biology 76:103326 PubMed39180984
2024
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International journal of molecular sciences 25: PubMed38542301
2024
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Molecular oncology 18:2212-2233 PubMed38425123
2024
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23216 PubMed37779422
2023
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Kidney360 4:1080-1094 PubMed37222594
2023
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