重组Anti-PDIA6抗体[EPR10132(B)]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody [EPR10132(B)] (AB154820)

Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PDIA6 with purified ab154820 at 1 : 100 dilution (9.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PDIA6 antibody [EPR10132(B)] (AB154820)

Flow Cytometry analysis of HT-1080 (Human fibrosarcoma epithelial cell) cells labelling PDIA6 with purified ab154820 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

• WB

Supplier Data

Western blot - Anti-PDIA6 antibody [EPR10132(B)] (AB154820)

All lanes:

Western blot - Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 3:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Rat spleen lysate at 20 µg

Lane 5:

Rat testis lysate at 20 µg

Lane 6:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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• WB

Lab

Western blot - Anti-PDIA6 antibody [EPR10132(B)] (AB154820)

Western blot : Anti-PDIA6 antibody [EPR10132(B)] ab154820 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 49 kDa in Wild-type A549 cell lysates with no signal observed at this size in PDIA6 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PDIA6 knockout A549 at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

HT1080 at 20 µg

Lane 5:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa,37 kDa

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