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重组RabMAb

重组Anti-PDIA2/PDIP抗体[EPR12212] (ab180931)

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Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
  • Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
  • Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12212] to PDIA2/PDIP
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PDIA2/PDIP抗体[EPR12212]
    参阅全部 PDIA2/PDIP 一抗

  • 描述

    兔单克隆抗体[EPR12212] to PDIA2/PDIP

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human pancreas, HeLa, BxPC-3 and HepG2 cell lysates; HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180931于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能

    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.

  • 组织特异性

    Highly expressed in pancreas (at protein level).

  • 序列相似性

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.

  • 翻译后修饰

    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.

  • 细胞定位

    Endoplasmic reticulum lumen.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

图片

  • Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
    Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)

    Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling PDIA2/PDIP with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

     

     

  • Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
    Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
    All lanes : Anti-PDIA2/PDIP antibody [EPR12212] (ab180931) at 1/20000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 58 kDa

  • Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
    Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)

文献 (0)

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