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Tags & Cell Markers Subcellular Markers Organelles ER

Anti-PDI1抗体[38H8] (ab4644)

  • Datasheet
Submit a review Q&A (4)References (4)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [38H8] to PDI1
  • Suitable for: ICC/IF, WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-PDI1抗体[38H8]
  • 描述

    小鼠单克隆抗体[38H8] to PDI1
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Saccharomyces cerevisiae
  • 免疫原

    Tissue, cells or virus corresponding to PDI1. Yeast nuclear preparations

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 纯化说明

    >90% pure based on SDS-PAGE analysis.
  • 克隆

    单克隆
  • 克隆编号

    38H8
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4644于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/500 - 1/5000.

(gives an ER staining pattern on yeast cells).

WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa).

Suggested working dilution of 1/5000 (see PMID: 17051412).

说明
ICC/IF
1/500 - 1/5000.

(gives an ER staining pattern on yeast cells).

WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa).

Suggested working dilution of 1/5000 (see PMID: 17051412).

靶标

  • 相关性

    PDI1 is a protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds.
  • 数据库链接

    • SwissProt: P17967 Saccharomyces cerevisiae
    • 别名

      • MFP1 antibody
      • PDI1 antibody
      • Pdi1p antibody
      • Protein disulfide isomerase antibody
      • Thioredoxin related glycoprotein 1 antibody
      • TRG1 antibody
      see all

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab4644?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4644 被引用在 4 文献中.

    • Vakirlis N  et al. De novo emergence of adaptive membrane proteins from thymine-rich genomic sequences. Nat Commun 11:781 (2020). PubMed: 32034123
    • Yi T  et al. LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission. Cell Death Dis 11:832 (2020). PubMed: 33028809
    • Delic M  et al. Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med 52:2000-12 (2012). WB . PubMed: 22406321
    • Damasceno LM  et al. Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Appl Microbiol Biotechnol 74:381-9 (2007). PubMed: 17051412

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Question

    According to a datasheet of ab4644, cellular localisation of PDI1 is ER and nuclear envelope. Have you ascertain the localisation in immune electron microscopy or western blotting of cytosolic fractions?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 20 2006

    Answer

    Thank you for your enquiry. I can confirm this antibody was screened by immunocytochemistry, selecting for various clones that stained yeast cells in an interesting and robust fashion. This particular antibody stains ER and the nuclear membrane, which is as expected for a PDI1p staining pattern. I am sorry we have no data on use of this antibody on various cytosolic fractions, but the antibody does work well in western blotting. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2006

    Question

    Thanks for your prompt reply.
    Find attached please the protein sequences (FASTA format) of P.
    pastoris PDI and KAR2 and the locus identification of each protein
    with which you can look for the other sequence format in Uniprot, for
    instance.
    I need antibodies against one of this proteins.
    Thanks for you help,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you for contacting us.

    Regrettably, we will not be able to guarantee the anti PDI antibodies cross reactivity towards P. pastoris species. The sequence analysis of ab30319 shows only 45% homology which is very less for antibody cross reactivity. I apologies for any inconvenience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Oct 17 2012

    Question

    Dear Sir/Madam Thank you for your reply. However I must say I am rather disappointed, as my expectations after the first call were not met. Also, our lab recently bought several other antibodies from you (therein ab6160 and ab8224, Ref. number: PM1118352) which also arrived at room temperature - even though I was assured this would not happen in the future. Also I see that my original inquiry was not included in the mail. Rather my second inquiry was referred to as the original one. Could the track have been lost somehow?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 12 2011

    Answer

    Thank you for your email. I apologize for your disappointment. I will try to explain what could have happen. The antibodies ab4644 and ab34772 were dispatched on 4th july and were delivered on 7th July. I am not sure if the person who received these antibodies kept these at 4C or I am also not sure for how long the antibodies were kept a room temperature. Again ab6160; ab8224 and ab10321 were dispatched on Sept. 2 and were delivered on Sept. 6. We cannot say the antibodies are getting damaged because they were at RT while shipment, because we have done nearly 13,000 individual ELISA experiments and we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this. As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. The ice pack may be thawed or even at room temperature when you receive it; please be assured that this is normal and that your product is safe to use. Once you have received the vial, please follow the long-term storage instructions on the datasheet. Our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. We also have not lost any information you have kindly provided while calling us. We normally do not write each and every part of conversation we discuss with our customers. However we always make sure we have enough information for understanding of problem. You have kindly contacted us regarding the inconsistent results you have observed. Inconsistent results can be due to many reasons, so in order to understand the reason I asked more details about this. I am sorry I did not receive any reply. I could only say that the antibodies must be tried and if they do not give expected results as stated on the datasheet and our scientific support team unable to troubleshoot the problem then we will be very happy to replace the products at no extra cost. I have attached the WB questionnaire; please fill in this and send it to us asap. We will then take a look at the protocol and will see if the protocol is OK and if it cannot be optimized then we will send free of charge replacement right away. Should you have any question please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Sep 12 2011

    Question

    I was wondering if you have any references for papers that have used this antibody for immunofluorescent studies Marcus

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2003

    Answer

    Unfortunately, we are not aware of any publications that feature the use of this antibody.

    Read More

    Abcam Scientific Support

    回复于 Dec 09 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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