Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证

Anti-PDHA1抗体[8D10E6] (ab110334)

Reviews (6)Q&A (5)References (70)
Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)

Key features and details

  • Mouse monoclonal [8D10E6] to PDHA1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Cow, Human, Drosophila melanogaster
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

HRP

概述

  • 产品名称

    Anti-PDHA1抗体[8D10E6]
    参阅全部 PDHA1 一抗

  • 描述

    小鼠单克隆抗体[8D10E6] to PDHA1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IF, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human, Drosophila melanogaster
    预测可用于: Zebrafish

  • 免疫原

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • 阳性对照

    • WB: Isolated mitochondria from human, bovine, rat and mouse heart. HepG2 (human liver hepatocellular carcinoma cell line) cell lysate. FACS: HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110334于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (6)
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal)
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal)
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • 组织特异性

    Ubiquitous.

  • 疾病相关

    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.

  • 细胞定位

    Mitochondrion matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    • Pyruvate Dehydrogenase E1-alpha subunit antibody
    see all

图片

  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    All lanes : Anti-PDHA1 antibody [8D10E6] (ab110334) at 0.5 µg/ml

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : PDHA1 knockout HeLa whole cell lysate
    Lane 3 : HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab110334 observed at 43 kDa. Red - loading control, ab52866, observed at 50 kDa.

    ab110334 was shown to recognize PDHA1 in wild-type HeLa cells as signal was lost at the expected MW in PDHA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. Ab110334 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 0.5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    All lanes : Anti-PDHA1 antibody [8D10E6] (ab110334) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)

    Immunocytochemistry analysis using ab110334 at 1 µg/ml staining PDHA1 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells (4% paraformaldehyde fixed and Triton X-100 permeabilized).
    The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).

  • Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)

    Flow cytometric analysis using ab110334 at 1 µg/ml staining PDHA1 in HL-60 (human promyelocytic leukemia cell line) cells (blue). Isotype control antibody (red).

文献 (70)

发表研究结果有使用 ab110334？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110334 被引用在 70 文献中.

  • Izzo LT  et al. Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. Sci Adv 9:eadf0115 (2023). PubMed: 37134161
  • Goyal M  et al. Blood progenitor redox homeostasis through olfaction-derived systemic GABA in hematopoietic growth control in Drosophila. Development 149:N/A (2022). PubMed: 34850846
  • Emtenani S  et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. EMBO J 41:e109049 (2022). PubMed: 35319107
  • Zhang Y  et al. Sirtuin 3 regulates mitochondrial protein acetylation and metabolism in tubular epithelial cells during renal fibrosis. Cell Death Dis 12:847 (2021). PubMed: 34518519
  • Duraj T  et al. Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma. Cells 10:N/A (2021). PubMed: 33498369
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Sheep Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alex Pendleton

Verified customer

提交于 Nov 14 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 01 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
HEK cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Euglena Cell lysate - whole cell (Euglena gracilis SM-ZK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Treatment
silencing of pyruvate dehydrogenase E1-alpha subunit or not
Specification
Euglena gracilis SM-ZK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 07 2016

Question

I would be interested in the offer of a discount after supplying an abreview. But I am just wondering if there is a possibility of getting a sample of this antibody first? As our lab has found in the past that some antibodies even if the sequence is quite homologous it does not always work and so I would be cautious about buying this antibody before it has been tested.

Kind Regards,

Read More
Answer

Thank you for contacting us.

We unfortunately do not provide free samples, this is why we have 100% Abtrial offer. If this antibody does not work you will still be getting a free antibody with Abtrial code.

Please let me know if you are interested in getting this code.

Thanks!

Read More

Question

Inquiry: Is it known whether this antibody works with horse samples? I am working with skeletal muscle isolated mitochondria and I am looking for a loading control antibody for western blotting. I have been using Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5 ] (ab110330) when using rat and mouse samples but I now need one that will work with horse also.

Read More
Answer

Thank you for contacting us.

This product ab110330 was tested with Mouse, Rat, Cow and Human samples only; horse was never tried. We can however predict, it will be fine for horse samples as well because the horse protein (http://www.uniprot.org/uniprot/F6WQM7) is quite homologous to the human protein (http://www.uniprot.org/uniprot/P08559).

In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

One person in our lab has two antibodies not working now:
" 1. anti-Pyruvate Dehydrogenase (Mouse monoclonal): Abcam (Catalog#
ab110334). Stored at 4 degrees with our other
antibodies.
2. anti-SDHA (Mouse monoclonal): Abcam (Catalog# ab14715).
Stored at 4 degrees with our other antibodies. Our other antibodies
(even those ordered much before than these) are working fine. I used
these antibodies 4 times for SDHA and 3 times for PDH on different
dates. So the probability of them being outside for prolonged period
and other physical insults is negligible. I think I am the only one in
the lab who uses these antibodies. so mishandling is also not an
issue."
Both of them worked in the beginning beautifully but when I revisited
them, they were not working.
Would you please help me with that? Please let me know if you have any
questions, thank you.

Read More
Answer

Thank you for contacting us. I am sorry that these antibodies are no longer working properly. I will be happy to help you resolve this issue if you can please provide some additional details about your experiment?

1.) What application were you using these antibodies in (WB, IHC-P, etc)?

2.) What species, tissue type, or cell line were you testing? How were the samples prepared (type of buffer used for lysis, fixation, permeabilization, embedding method, etc)?

3.) What blocking agent was used and at what concentration?

4.) What were the primary and secondary antibody dilutions and incubation times?

5.) Could you please provide a description of the problem (no signal, high background, etc) and / or an image of your results?

6.) What was your original order or PO number?

I will be happy to help you further once I have this additional information.

Read More

Question

One person in our lab has two antibodies not
working now:
" 1. anti-Pyruvate Dehydrogenase (Mouse monoclonal): Abcam (Catalog#
ab110334) Lot# GR47055-1. Stored at 4 degrees with our other
antibodies.
2. anti-SDHA (Mouse monoclonal): Abcam (Catalog# ab14715) Lot# 888711.
Stored at 4 degrees with our other antibodies. Our other antibodies
(even those ordered much before than these) are working fine. I used
these antibodies 4 times for SDHA and 3 times for PDH on different
dates. So the probability of them being outside for prolonged period
and other physical insults is negligible. I think I am the only one in
the lab who uses these antibodies. so mishandling is also not an
issue."
Both of them worked in the beginning beautifully but when I revisited
them, they were not working.
Would you please help me with that? Please let me know if you have any
questions, thank you.

Read More
Answer

Thank you for contacting us.

I found the original order for these ordered on July 28 2011. Since these antibodies are recommended to be stored at 4C, they only have a shelf-life of roughly 1 yr. If the end-user is seeing decreased signal and the antibodies worked well in the past they may have to increase the working dilution and/or incubation time.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com