重组Anti-PDGFR beta (phospho Y857)抗体[EP2136Y] (ab62367)
![Western blot - Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)](https://www.abcam.cn/ps/products/62/ab62367/Images/ab62367wb.gif "Western blot - Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2136Y] to PDGFR beta (phospho Y857)
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-PDGFR beta (phospho Y857)抗体[EP2136Y]
参阅全部 PDGFR beta 一抗 -
描述
兔单克隆抗体[EP2136Y] to PDGFR beta (phospho Y857) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- NIH3T3 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2136Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62367于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 123 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 123 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
疾病相关
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 18596 Mouse
- SwissProt: P05622 Mouse
- Unigene: 4146 Mouse
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别名
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
图片
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Western blot - Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)All lanes : Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysates (untreated)
Lane 2 : NIH3T3 cell lysates treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit, HRP labeled, at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
![Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)](https://www.abcam.cn/ps/products/62/ab62367/Images/ab62367-2-benefits-of-recombinant-antibodies.png)
Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62367 被引用在 1 文献中.
- Efthymiou V et al. Inhibition of AXL receptor tyrosine kinase enhances brown adipose tissue functionality in mice. Nat Commun 14:4162 (2023). PubMed: 37443109