Anti-PDGFR beta抗体[42G12] (ab69506)
Key features and details
- Mouse monoclonal [42G12] to PDGFR beta
- Suitable for: Indirect ELISA, IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-PDGFR beta抗体[42G12]
参阅全部 PDGFR beta 一抗 -
描述
小鼠单克隆抗体[42G12] to PDGFR beta -
宿主
Mouse -
经测试应用
适用于: Indirect ELISA, IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human PDGFR beta.
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阳性对照
- IHC-P: Human spleen and placenta tissues; Mouse heart muscle lysate. WB: Wild-type SH-SY5Y cell lysate. Flow Cyt: NIH 3T3 cells. ELISA: Human PDGFR beta protein
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常规说明
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
42G12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69506于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Indirect ELISA |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Indirect ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
疾病相关
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5159 Human
- Entrez Gene: 18596 Mouse
- Omim: 173410 Human
- SwissProt: P09619 Human
- SwissProt: P05622 Mouse
- Unigene: 509067 Human
- Unigene: 4146 Mouse
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别名
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)
Immunohistochemistry analysis of human spleen tissue labeling PDGFR beta [42G12] with ab69506 at 20µg/ml.
This image was generated using the ascites version of the product.
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All lanes : Anti-PDGFR beta antibody [42G12] (ab69506) at 1/1000 dilution
Lane 1 : Wild-type SH-SY5Y cell lysate
Lane 2 : PDGFRB knockout SH-SY5Y cell lysate
Lane 3 : Human Skeletal Muscle tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 124 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab69506 observed at 170 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab69506 was shown to react with PDGFR beta in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab69506 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [42G12] (ab69506)
Immunohistochemistry analysis of human placenta tissue labeling PDGFR beta [42G12] with ab69506 at at 20µg/ml.
This image was generated using the ascites version of the product.
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Overlay histogram showing NIH 3T3 cells stained with ab69506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69506, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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ELISA using ab69506 at varying antibody concentrations (1000-0ng/ml). The antigens used were Human PDGFR alpha protein, Human PDGFR beta protein at a concentration of 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) was used as the secondary antibody with a dilution of 1:1000. The substrate solution used was p-nitrophenyl phosphate(PNPP)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab69506 被引用在 44 文献中.
- Zhang Y et al. Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells. Commun Biol 6:476 (2023). PubMed: 37127734
- Xie J et al. Clearance of Stress-Induced Premature Senescent Cells Alleviates the Formation of Abdominal Aortic Aneurysms. Aging Dis 14:1778-1798 (2023). PubMed: 37196124
- Wang H et al. 7-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence and Fibrosis of Choroidal Neovascularization via IQGAP1 Phosphorylation-Dependent Signaling. Int J Mol Sci 24:N/A (2023). PubMed: 37373423
- Gu X et al. TNFSF15 facilitates the differentiation of CD11b+ myeloid cells into vascular pericytes in tumors. Cancer Biol Med 20:869-84 (2023). PubMed: 37921408
- Guo S et al. A Preliminary Study on the Correlation Between Age and Endometrial Receptivity. Pharmgenomics Pers Med 16:425-432 (2023). PubMed: 37180957