Anti-PDGFR alpha (phospho Y742)抗体(ab5452)
Key features and details
- Rabbit polyclonal to PDGFR alpha (phospho Y742)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
-
产品名称
Anti-PDGFR alpha (phospho Y742)抗体
参阅全部 PDGFR alpha 一抗 -
描述
兔多克隆抗体to PDGFR alpha (phospho Y742) -
宿主
Rabbit -
特异性
PDGFR beta (50% homologous) has not been tested, but is not expected to react. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human, Xenopus laevis
-
免疫原
Synthetic peptide corresponding to Human PDGFR alpha (phospho Y742).
-
阳性对照
- NIH3T3 cells +/- PDGF.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosine 742. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170-185 kDa.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170-185 kDa. |
靶标
-
功能
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
组织特异性
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
疾病相关
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
- SwissProt: P26618 Mouse
- SwissProt: P20786 Rat
- SwissProt: P26619 Xenopus laevis
see all -
别名
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
图片
-
Western blot - Anti-PDGFR alpha (phospho Y742) antibody (ab5452)Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50
µ g/mL ab5452 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5452 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5)
实验方案
数据表及文件
-
Datasheet download
文献 (6)
ab5452 被引用在 6 文献中.
- Shao M et al. Pathologic HIF1α signaling drives adipose progenitor dysfunction in obesity. Cell Stem Cell 28:685-701.e7 (2021). PubMed: 33539723
- Bjune JI et al. The homeobox factor Irx3 maintains adipogenic identity. Metabolism 103:154014 (2020). PubMed: 31751577
- Røsland GV et al. Epithelial to mesenchymal transition (EMT) is associated with attenuation of succinate dehydrogenase (SDH) in breast cancer through reduced expression of SDHC. Cancer Metab 7:6 (2019). PubMed: 31164982
- Ip CKM et al. Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies. Nat Commun 9:4583 (2018). PubMed: 30389923
- Paugh BS et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res 73:6219-29 (2013). Human . PubMed: 23970477
- Rao RC et al. Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures. Stem Cells : (2008). WB ; Mouse . PubMed: 18403757
