重组Anti-PDGFR alpha (phospho Y720)抗体[EP2478] (ab134068)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2478] to PDGFR alpha (phospho Y720)
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-PDGFR alpha (phospho Y720)抗体[EP2478]
参阅全部 PDGFR alpha 一抗 -
描述
兔单克隆抗体[EP2478] to PDGFR alpha (phospho Y720) -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: NIH/3T3 treated with 100nM Pervanadate. ICC/IF: NIH/3T3 cells. Flow Cyt (Intra): NIH/3T3 cells.
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常规说明
This product has switched from a hybridoma to recombinant production method on 31st May 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2478 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000. Predicted molecular weight: 190 kDa.
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|
Flow Cyt (Intra) |
1/1000.
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ICC/IF |
1/100.
|
说明 |
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WB
1/10000. Predicted molecular weight: 190 kDa. |
Flow Cyt (Intra)
1/1000. |
ICC/IF
1/100. |
靶标
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功能
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
组织特异性
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
疾病相关
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
- SwissProt: P26618 Mouse
- SwissProt: P20786 Rat
- Unigene: 74615 Human
see all -
别名
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
图片
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All lanes : Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/10000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate, then membrane treated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 190 kDa
Observed band size: 190 kDa
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PDGFR alpha with ab134068 at 1/100 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of NIH/3T3 cells. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PDGFR alpha with ab134068 at 1/1000 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab134068 被引用在 4 文献中.
- Zhong J et al. Circular RNA encoded MET variant promotes glioblastoma tumorigenesis. Nat Commun 14:4467 (2023). PubMed: 37491377
- Sanada TJ et al. Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. PLoS One 14:e0214654 (2019). PubMed: 30925179
- Stivala S et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest 130:1596-1611 (2019). PubMed: 30730307
- Kiseleva AA et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs. Clin Cancer Res 25:4179-4193 (2019). PubMed: 30867219