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使用敲除细胞株进行验证重组RabMAb

重组Anti-PDGFR alpha抗体[EPR22059-270] (ab203491)

  • Datasheet
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  • Certificate of Compliance
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Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunoprecipitation - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Indirect ELISA - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Frozen sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
  • Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22059-270] to PDGFR alpha
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP, Indirect ELISA, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-PDGFR alpha抗体[EPR22059-270]
    参阅全部 PDGFR alpha 一抗
  • 描述

    兔单克隆抗体[EPR22059-270] to PDGFR alpha
  • 宿主

    Rabbit
  • 特异性

    PDGFR alpha is membrane protein, so enrichment of membrane could help increasing the detection level of PDGFR alpha.

  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, Flow Cyt, IP, Indirect ELISA, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: SH-SY5Y, A-204, MG-63, C6, and NIH/3T3 whole cell lysates. IHC-P: Mouse E14.5 lung, E14.5 intervertebral disc and uterus tissues; Rat E14.5 intervertebral disc tissue and Human endometrium tissue. ICC/IF: SH-SY5Y and A-204 cells. Flow Cyt: NIH/3T3 and A-204 cells. IP: A-204 whole cell lysate IHC-Fr: Human kidney tissue sections.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR22059-270
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Cancer
    • Growth factors
    • PDGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Development

相关产品

  • Alternative Versions

    • Anti-PDGFR alpha antibody [EPR22059-270] - BSA and Azide free (ab234965)
    • Alexa Fluor® 647 Anti-PDGFR alpha antibody [EPR22059-270] (ab270085)
    • PE Anti-PDGFR alpha antibody [EPR22059-270] (ab270086)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PDGFRA knockout SH-SY5Y cell line (ab275335)
  • KO cell lysates

    • Human PDGFRA knockout SH-SY5Y cell lysate (ab275522)
  • Recombinant Protein

    • Recombinant human PDGFR alpha protein (ab84797)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab203491于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 150, 180 kDa (predicted molecular weight: 122 kDa).
IHC-P (1)
Use a concentration of 0.26 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (1)
1/500.
Flow Cyt
1/50.
IP
1/30.
Indirect ELISA
Use a concentration of 1 µg/ml.
IHC-Fr (1)
1/500.
说明
WB
1/1000. Detects a band of approximately 150, 180 kDa (predicted molecular weight: 122 kDa).
IHC-P
Use a concentration of 0.26 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
Flow Cyt
1/50.
IP
1/30.
Indirect ELISA
Use a concentration of 1 µg/ml.
IHC-Fr
1/500.

靶标

  • 功能

    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 组织特异性

    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 疾病相关

    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P16234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5156 Human
    • Entrez Gene: 18595 Mouse
    • Entrez Gene: 25267 Rat
    • Omim: 173490 Human
    • SwissProt: P16234 Human
    • SwissProt: P26618 Mouse
    • SwissProt: P20786 Rat
    • Unigene: 74615 Human
    • Unigene: 221403 Mouse
    • Unigene: 55127 Rat
    see all
  • 别名

    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

图片

  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Lanes 1-8 : Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) at 1/1000 dilution
    Lanes 9-12 : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lanes 1 & 5 & 9 : SH-SY5Y
    Lanes 2 & 6 & 10 : Human brain tissue lysate at 20 µg
    Lanes 3 & 7 & 11 : Human heart tissue lysate at 20 µg
    Lanes 4 & 8 & 12 : Human lung tissue lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 80 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    We recommend using higher sensitivity ECL to improve results.

    ab134123 can be a good alternative when testing samples with low level of PDGFR alpha which detects stronger signal than ab203491 in western blot.

  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Lanes 1-10 : Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) at 1/1000 dilution
    Lanes 11-15 : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lanes 1 & 6 & 11 : Rat heart tissue lysate
    Lanes 2 & 7 & 12 : Rat lung tissue lysate
    Lanes 3 & 8 & 13 : Mouse brain tissue lysate
    Lanes 4 & 9 & 14 : Mouse heart tissue lysate
    Lanes 5 & 10 & 15 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 80 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    We recommend using higher sensitivity ECL to improve results.

    ab134123 can be a good alternative when testing samples with low level of PDGFR alpha which detects stronger signal than ab203491 in western blot.

  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    All lanes : Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y cell lysate
    Lane 2 : PDGFRA knockout SH-SY5Y cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab203491 observed at 150 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab203491 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab203491 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded human human bladder carcinoma labeling PDGFR alpha with ab203491 at 0.26 µg/mL followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human bladder carcinoma was observed. The section was incubated with ab203491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PDGFR alpha with ab203491 at 0.26 µg/mL followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human endometrium was observed. The section was incubated with ab203491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    ab203491 staining PDGFR alpha in wild-type SH-SY5Y cells (top panel) and PDGFRA knockout SH-SY5Y cells (ab275335) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab203491 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunocytochemistry/ Immunofluorescence - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma cell line) cells and A-172 (human brain glioblastoma cell line) labeling PDGFR alpha with ab203491 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membranous and cytoplasmic staining in A-204 cell line.

    Negative control: A-172 (PMID:8425771.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    All lanes : Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 150,180 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lane 1: 3 minutes; Lane 2: 59 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on interstitial cells of mouse uterus (PMID: 25788664). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunoprecipitation - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunoprecipitation - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    PDGFR alpha was immunoprecipitated from 0.35 mg of A-204 (human muscle rhabdomyosarcoma cell line) whole cell lysate with ab203491 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203491 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: A-204 whole cell lysate 10 µg (Input). 

    Lane 2: ab203491 IP in A-204 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203491 in A-204 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds.

  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Flow cytometric analysis of NIH/3T3 (mouse embyro fibroblast cell line) cell line labeling PDGFR alpha with ab203491 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Indirect ELISA - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Indirect ELISA - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Indirect ELISA using ab203491 at varying antibody concentrations (4000~0 ng /ml) and Human PDGF Receptor alpha antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution dilution was used as a secondary antibody.
  • Immunohistochemistry (Frozen sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Frozen sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    IHC image of PDGFR alpha staining in a section of frozen normal human kidney performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab203491, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 intervertebral disc tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in mesenchymal cells of rat E14.5 intervertebral disc (PMID: 9199674). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 lung tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the mesenchyme of mouse E14.5 lung (PMID: 8681381). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 intervertebral disc tissue labeling PDGFR alpha with ab203491 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in mesenchymal cells of mouse E14.5 intervertebral disc (PMID: 9199674). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    All lanes : Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) at 1/1000 dilution

    Lane 1 : A-204 (Human muscle rhabdomyosarcoma cell line) whole cell lysate
    Lane 2 : MG-63 (human osteosarcoma fibroblast cell line) whole cell lysate
    Lane 3 : A-172 (human brain glioblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 150,180 kDa why is the actual band size different from the predicted?


    Exposure time: 92 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Flow Cytometry - Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

    Flow cytometric analysis of A-172 (human brain glioblastoma cell line, left) and A-204 (human muscle rhabdomyosarcoma cell line, right) cell lines labeling PDGFR alpha with ab203491 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)
    Anti-PDGFR alpha antibody [EPR22059-270] (ab203491)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

文献 (22)

发表研究结果有使用 ab203491?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab203491 被引用在 22 文献中.

  • Sosa BR  et al. A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells. JBMR Plus 6:e10596 (2022). PubMed: 35309866
  • Chen L  et al. Remodeling of dermal adipose tissue alleviates cutaneous toxicity induced by anti-EGFR therapy. Elife 11:N/A (2022). PubMed: 35324426
  • Bo-Yin Z  et al. Unlocking the Recovery Potential: JMJD3 Inhibition-Mediated SAPK/JNK Signaling Inactivation Supports Endogenous Oligodendrocyte-Lineage Commitment Post Mammalian Spinal Cord Injury. Neurochem Res 46:792-803 (2021). PubMed: 33428096
  • Zhang J  et al. miR-224 aggravates cancer-associated fibroblast-induced progression of non-small cell lung cancer by modulating a positive loop of the SIRT3/AMPK/mTOR/HIF-1a axis. Aging (Albany NY) 13:10431-10449 (2021). PubMed: 33819917
  • Xia S  et al. Delta-like 4 is required for pulmonary vascular arborization and alveolarization in the developing lung. JCI Insight 6:N/A (2021). PubMed: 33830085
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-PDGFR alpha antibody [EPR22059-270]

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Various)
Permeabilization
Yes - 1% triton x
Specification
Various
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 02 2023

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody [EPR22059-270]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal Carcinoma (CRC))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9 IHC Antigen Retrieval Solution (Invitrogen high pH 00-4956-58)
Permeabilization
No
Specification
Colorectal Carcinoma (CRC)
Blocking step
protein block - serum free 0.25% casein in PBS, containing stabilizing protein and 0.015 mol/L sodium azide as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Amy Burley

Verified customer

提交于 Dec 22 2022

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PDGFR alpha antibody [EPR22059-270]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 15min PBST
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Nicole Brossier

Verified customer

提交于 Jul 26 2022

Immunohistochemistry (Frozen sections) abreview for Anti-PDGFR alpha antibody [EPR22059-270]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Skeletal muscle)
Permeabilization
No
Specification
Skeletal muscle
Blocking step
TritonX-100 as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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