Anti-PDGF B抗体[MM0014-5F66] (ab51869)
![Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)](https://www.abcam.cn/ps/products/51/ab51869/Images/ab51869-471133-anti-pdgf-b-antibody-mm0014-5f66-western-blot-placenta-human.jpg "Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)")
Key features and details
- Mouse monoclonal [MM0014-5F66] to PDGF B
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-PDGF B抗体[MM0014-5F66]
参阅全部 PDGF B 一抗 -
描述
小鼠单克隆抗体[MM0014-5F66] to PDGF B -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein (Human)
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阳性对照
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue. Flow Cyt: SHSY-5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MM0014-5F66 -
同种型
IgG1 -
研究领域
相关产品
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51869于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 27 kDa.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/1000. Predicted molecular weight: 27 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
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别名
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
图片
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Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)Anti-PDGF B antibody [MM0014-5F66] (ab51869) at 1/1000 dilution + Human placenta tissue lysate
Predicted band size: 27 kDa
Observed band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)](https://www.abcam.cn/ps/products/51/ab51869/Images/ab51869-63939-anti-pdgf-bb-antibody-mm0014-5f66-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)ab51869 at 1/200 dilution, staining PDGF BB in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Antigen retrieval was done using proteinase K (20 µg/mL in PBS: Room temperature, ranging from 5 min to 30 mins).
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![Flow Cytometry - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)](https://www.abcam.cn/ps/products/51/ab51869/Images/ab51869-63938-anti-pdgf-bb-antibody-mm0014-5f66-flow-cytometry.jpg)
Flow Cytometry - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)Overlay histogram showing SHSY-5Y cells stained with ab51869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51869, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab51869 被引用在 6 文献中.
- Li Y et al. Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer. Theranostics 12:3818-3833 (2022). PubMed: 35664061
- Xie G et al. Conjugated secondary 12a-hydroxylated bile acids promote liver fibrogenesis. EBioMedicine 66:103290 (2021). PubMed: 33752128
- Silva CR et al. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater 77:155-171 (2018). PubMed: 30031163
- Miyata T et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P, ICC/IF ; Human . PubMed: 24512807
- Niu F et al. Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders. J Neurosci 34:11812-25 (2014). IHC-P, ICC/IF . PubMed: 25164676
- Suzuki S et al. Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor-ß phosphorylation, and microvessel density in gastric cancer. BMC Cancer 10:659 (2010). WB ; Human . PubMed: 21118571