Anti-PDE8B抗体(ab112024)
Key features and details
- Rabbit polyclonal to PDE8B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDE8B抗体
参阅全部 PDE8B 一抗 -
描述
兔多克隆抗体to PDE8B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within C terminal amino acids 835-885 of Human PDE8B (NP_003710.1)
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阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112024于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland. -
组织特异性
Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates. -
通路
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
疾病相关
Defects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD) [MIM:609161]. It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8622 Human
- Omim: 603390 Human
- SwissProt: O95263 Human
- Unigene: 584830 Human
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别名
- 3' 5' cyclic nucleotide phosphodiesterase 8B antibody
- 3'5' cyclic nucleotide phosphodiesterase 8B antibody
- Cell proliferation-inducing gene 22 protein antibody
see all
图片
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Immunoprecipitation - Anti-PDE8B antibody (ab112024)Immunoprecipitation of PDE8B. Samples: Whole cell lysate from HeLa cells. 20% of IP loaded. Antibodies: Affinity purified rabbit anti-PDE8B antibody (ab112024) at 1 µg/ml. Lane 1- PDE8B was immunoprecipitated by rabbit anti-PDE8B antibody (ab112024) at 6 mcg/mg lysate. Lane 2- PDE8B was immunoprecipitated by an rabbit anti-PDE8B antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.
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Western blot - Anti-PDE8B antibody (ab112024)All lanes : Anti-PDE8B antibody (ab112024) at 0.4 µg/ml
Lane 1 : Whole cell extracts from HeLa cells at 50 µg
Lane 2 : Whole cell extracts from HeLa cells at 15 µg
Lane 3 : Whole cell extracts from HeLa cells at 5 µg
Predicted band size: 99 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab112024 尚未被引用在任何文献中。