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AB5659

Anti-PDE6 alpha抗体

Anti-PDE6 alpha antibody

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(14 Publications)

Rabbit Polyclonal PDE6 alpha antibody. Suitable for IHC-P, WB and reacts with Human, Sheep samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human PDE6A aa 1-50.

查看别名

PDEA, PDE6A, GMP-PDE alpha, PDE V-B1

2 Images
Western blot - Anti-PDE6 alpha antibody (AB5659)
  • WB

Unknown

Western blot - Anti-PDE6 alpha antibody (AB5659)

Western blot of PDE6 alpha on sheep retinal extracts using ab5659.

All lanes:

Western blot - Anti-PDE6 alpha antibody (ab5659)

Predicted band size: 100 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)

IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Sheep, Human

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PDE6A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16499

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.00000-2.00000 µg/mL", "WB-species-notes": "<p>representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. See Guo et al, Kerov et al for details of WB procedure.</p>" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 6 alpha (PDE6 alpha) is an enzyme that plays a significant role in the phototransduction pathway. It is also known as PDE6A and has a molecular mass of approximately 100 kDa. This enzyme is highly expressed in the rod and cone cells of the retina acting as a component in the conversion of light signals into visual information. PDE6 alpha hydrolyzes cyclic GMP into 5'-GMP decreasing the intracellular levels of cGMP. This action is critical in regulating the conductance of ion channels in photoreceptor cells.
Biological function summary

PDE6 alpha functions within a larger enzyme complex known as the PDE6 holoenzyme. This complex consists of multiple subunits including the PDE6 beta and PDE6 gamma subunits which form the catalytic core. PDE6 alpha contributes to the regulation of cGMP levels which is essential for maintaining the electrical response of photoreceptors to light. The precise action of hydrolyzing cGMP mediates the phototransduction cascade that eventually leads to visual perception.

Pathways

With regard to pathways in cellular function PDE6 alpha is an important player in the phototransduction and visual signal transduction pathways. Within the phototransduction pathway PDE6 alpha interacts closely with other proteins like rhodopsin and transducin. These proteins activate PDE6 alpha upon light exposure initiating the cascade that results in hyperpolarization of the photoreceptor cell membrane. The role of PDE6 alpha in managing cGMP concentration positions it as a major component in these pathways.

Mutations in the genes encoding PDE6 alpha have been associated with retinitis pigmentosa and congenital stationary night blindness. Retinitis pigmentosa a progressive degenerative disease of the retina often involves the dysfunction of photoreceptor cells where altered PDE6 alpha function impacts the phototransduction process. Additionally PDE6 alpha's involvement in these disorders also connects it to proteins such as arrestin and recoverin which further influence the progression of these visual impairments.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Rod-specific cGMP phosphodiesterase that catalyzes the hydrolysis of 3',5'-cyclic GMP (PubMed : 20940301). This protein participates in processes of transmission and amplification of the visual signal.
See full target information PDE6A

文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:20195 PubMed36424523

2022

Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.

Applications

Unspecified application

Species

Unspecified reactive species

Vyara Todorova,Luca Merolla,Duygu Karademir,Gabriele M Wögenstein,Julian Behr,Lynn J A Ebner,Marijana Samardzija,Christian Grimm

Cellular and molecular life sciences : CMLS 78:6505-6532 PubMed34420069

2021

A look into retinal organoids: methods, analytical techniques, and applications.

Applications

Unspecified application

Species

Unspecified reactive species

Tess A V Afanasyeva,Julio C Corral-Serrano,Alejandro Garanto,Ronald Roepman,Michael E Cheetham,Rob W J Collin

The Journal of biological chemistry 291:10501-14 PubMed27008858

2016

Mfsd2a Is a Transporter for the Essential ω-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.

Applications

Unspecified application

Species

Unspecified reactive species

Bernice H Wong,Jia Pei Chan,Amaury Cazenave-Gassiot,Rebecca W Poh,Juat Chin Foo,Dwight L A Galam,Sujoy Ghosh,Long N Nguyen,Veluchamy A Barathi,Sia W Yeo,Chi D Luu,Markus R Wenk,David L Silver

Cell communication and signaling : CCS 12:67 PubMed25323447

2014

Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina.

Applications

WB

Species

Mouse

Shanta Sarfare,Alex S McKeown,Jeffrey Messinger,Glen Rubin,Hongjun Wei,Timothy W Kraft,Steven J Pittler

Nature communications 5:4047 PubMed24915161

2014

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufeng Zhong,Christian Gutierrez,Tian Xue,Christopher Hampton,M Natalia Vergara,Li-Hui Cao,Ann Peters,Tea Soon Park,Elias T Zambidis,Jason S Meyer,David M Gamm,King-Wai Yau,M Valeria Canto-Soler

Human molecular genetics 23:514-23 PubMed24101599

2013

Mid-stage intervention achieves similar efficacy as conventional early-stage treatment using gene therapy in a pre-clinical model of retinitis pigmentosa.

Applications

WB

Species

Human

Katherine J Wert,Javier Sancho-Pelluz,Stephen H Tsang

Human molecular genetics 22:558-67 PubMed23108158

2012

Gene therapy provides long-term visual function in a pre-clinical model of retinitis pigmentosa.

Applications

WB

Species

Mouse

Katherine J Wert,Richard J Davis,Javier Sancho-Pelluz,Patsy M Nishina,Stephen H Tsang

Proceedings of the National Academy of Sciences of 106:17534-9 PubMed19805139

2009

Retinoid-related orphan nuclear receptor RORbeta is an early-acting factor in rod photoreceptor development.

Applications

IHC-FoFr, WB

Species

Mouse, Mouse

Li Jia,Edwin C T Oh,Lily Ng,Maya Srinivas,Matthew Brooks,Anand Swaroop,Douglas Forrest

The Journal of neuroscience : the official journal of the Society for Neuroscience 29:9748-60 PubMed19657028

2009

Essential and synergistic roles of RP1 and RP1L1 in rod photoreceptor axoneme and retinitis pigmentosa.

Applications

WB

Species

Mouse

Tetsuji Yamashita,Jiewu Liu,Jiangang Gao,Sean LeNoue,Changguan Wang,Jack Kaminoh,Sara J Bowne,Lori S Sullivan,Stephen P Daiger,Kang Zhang,Malinda E C Fitzgerald,Vladimir J Kefalov,Jian Zuo

The Journal of biological chemistry 280:41069-76 PubMed16207703

2005

Transducin activation state controls its light-dependent translocation in rod photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Vasily Kerov,Desheng Chen,Mustapha Moussaif,Yu-Jiun Chen,Ching-Kang Chen,Nikolai O Artemyev
View all publications

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