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Anti-PDE4D (phospho S190)抗体(ab59212)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-PDE4D (phospho S190) antibody (ab59212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (ab59212)

Key features and details

  • Rabbit polyclonal to PDE4D (phospho S190)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PDE4D (phospho S190)抗体
    参阅全部 PDE4D 一抗

  • 描述

    兔多克隆抗体to PDE4D (phospho S190)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human PDE4D aa 150-250 (phospho S190). (R-E-SP-F-L).
    Database link: Q08499

  • 阳性对照

    • IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    ab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59212于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).
说明
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).

靶标

  • 功能

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

  • 组织特异性

    Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

  • 通路

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

  • 疾病相关

    Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.

  • 序列相似性

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

  • 翻译后修饰

    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

  • 细胞定位

    Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

图片

  • Western blot - Anti-PDE4D (phospho S190) antibody (ab59212)
    Western blot - Anti-PDE4D (phospho S190) antibody (ab59212)
    All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution

    Lane 1 : K562 cell extract treated
    with H2O2 (100uM, 30mins)
    Lane 2 : K562 cell extract treated
    with H2O2 (100uM, 30mins) with immunising peptide

    Predicted band size: 91 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (ab59212)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (ab59212)
    ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

文献 (2)

发表研究结果有使用 ab59212？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab59212 被引用在 2 文献中.

  • Wilson NM  et al. Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. Front Syst Neurosci 10:5 (2016). WB . PubMed: 26903822
  • Oliva AA  et al. Phosphodiesterase isoform-specific expression induced by traumatic brain injury. J Neurochem 123:1019-29 (2012). PubMed: 23057870

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-PDE4D (phospho S190) antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Loading amount
25 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 21 2012

Question

Customer inquiring about testing ab59212 in mouse via western blot. Specifically inquiring about homology between human and mouse PDE4D protein.

Read More
Answer

Thank you for contacting Abcam.

We talked earlier on the phone about testing ab59212 in mouse via western blot. The mouse and human PDE4D protein have 98.5% homology; thus, this antibody is eligible for testing through our AbTrial program. Details about the program are given below:

To our knowledge, ab59212 has not been tested in mouse. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

We also discussed our RabMab promotion, which entitles you to a free rabbit monoclonal antibody with purchase of a primary antibody. Further information about this promotion is given below:

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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