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Anti-PDE4D抗体(ab99409)

Submit a review Q&A (1)
Western blot - Anti-PDE4D antibody (ab99409)
  • Immunoprecipitation - Anti-PDE4D antibody (ab99409)

Key features and details

  • Rabbit polyclonal to PDE4D
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PDE4D抗体
    参阅全部 PDE4D 一抗

  • 描述

    兔多克隆抗体to PDE4D

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • 免疫原

    Corresponds to a region between amino acids 709-759 of Human PDE4D (NP_001098101.1).

  • 阳性对照

    • HeLa whole cell lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab99409于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP
Use at 2-5 µg/mg of lysate.
说明
WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP
Use at 2-5 µg/mg of lysate.

靶标

  • 功能

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

  • 组织特异性

    Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

  • 通路

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

  • 疾病相关

    Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.

  • 序列相似性

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

  • 翻译后修饰

    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

  • 细胞定位

    Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
      • DKFZp686M11213 antibody
      • 5''-cyclic phosphodiesterase 4D antibody
      • cAMP specific phosphodiesterase 4D antibody
      • cAMP specific phosphodiesterase PDE4D6 antibody
      • cAMP-specific 3'' antibody
      • DPDE3 antibody
      • Dunce like phosphodiesterase E3 antibody
      • FLJ97311 antibody
      • HSPDE4D antibody
      • PDE43 antibody
      • Pde4d antibody
      • PDE4D_HUMAN antibody
      • PDE4DN2 antibody
      • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
      • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
      • Phosphodiesterase 4D cAMP specific antibody
      • Phosphodiesterase 4D, cAMP specific (dunce) antibody
      • STRK1 antibody
      see all

    图片

    • Western blot - Anti-PDE4D antibody (ab99409)
      Western blot - Anti-PDE4D antibody (ab99409)
      All lanes : Anti-PDE4D antibody (ab99409) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 91 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-PDE4D antibody (ab99409)
      Immunoprecipitation - Anti-PDE4D antibody (ab99409)
      ab99409 at 1µg/ml detecting PDE4D in HeLa whole cell lysate by WB following IP.

      Lane 1: ab99409 at 3µg/mg of lysate
      Lane 2: IP with an antibody which recognizes an downstream epitope of PDE4D
      Lane 3: control IgG.

      In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    文献 (0)

    发表研究结果有使用 ab99409？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab99409 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Hi,
    I wonder if you could give me some advice please.
    I want to purchase an antibody which can be used to detect ALL 9isoforms ofPDE4D (phosphodiesterase type 4D). From what I can gather, you have 16 antibodies for PDE4D, I have purchased one previously (ab14613) but this is only specific toisoforms PDE4D1, PDE4D2, PDE4D3,PDE4D4 and PDE4D5.
    The only other which appears to be similar to what I'm looking for is ab59249, whichstates "This antibody detects endogenous levels of totalPDE4D protein". However, when looking further down the page at the images, there appears to be only one band in the resulting WB. This is strange since that of the 9 isoforms, 6 are long and 3 are short i.e. I would expect to see 2 bands on the WB (as you do with ab14613).
    Please could you explain the situation and also advise on which antibody I would be best purchasing?
    Kind Regards,

    Read More
    Answer

    Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
    Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q08499).
    This antibody binds to isoforms 4, 3, 5, N3, 8, 9, 7, and 12. It does not bind to isoform 10, 1, 2, and 6. According to the isoforms that this antibody binds, you would expect to see two bands, approximately 25 and 85 kDa. Because the western blot on our website is cut off at 34 kDa, we can retest this antibody to see if it does actually bind the lower isoform as well.
    Although we haven’t specifically tested the antibody against all isoforms, there are other alternatives that may also recognise several forms of the protein:
    For ab77080 we ran a BLAST search and found the antigen sequence in all of 9 isoforms:
    https://www.abcam.com/PDE4D-antibody-ab77080.html
    Ab112018 also shares 100% identity to isoforms PDE4D1-9:
    https://www.abcam.com/PDE4D-antibody-ab112018.html
    Ab99409 and ab87329 recognize 10 of 12 isoforms listed for SwissProt entry Q08499. The two isoforms listed for SwissProt Q08499 not recognized by either of them are sp|Q08499-7|PDE4D_HUMAN and sp|Q08499-12|PDE4D_HUMAN:
    http://www.uniprot.org/uniprot/Q08499
    And ab87329’s immunogen is present in 8 out of 12 of them so it should detect isoforms 1- 10.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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