Anti-PDE4D抗体

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D antibody (AB14613)

ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• WB

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Western blot - Anti-PDE4D antibody (AB14613)

Western blot using ab14613 and positive controls for PDE4D variants.

All lanes:

Western blot - Anti-PDE4D antibody (ab14613)

Predicted band size: 91 kDa

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• WB

CiteAb

Western blot - Anti-PDE4D antibody (AB14613)

PDE4D western blot using anti-PDE4D antibody ab14613. Publication image and figure legend from Fang, Q., Ma, Y., et al., 2013, Mediators Inflamm, PubMed 24227907.

ab14613 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab14613 please see the product overview.

Effect of PGE2 and indomethacin on PDE4 expression. Confluent HFL-1 cells were treated with SF-DMEM with or without 10-9 M PGE2 or 10-6 M indomethacin for 24 hrs. Whole cell lysates were immunoblotted for PDE4 isoforms and β-actin as loading control. Images were visualized with ECL plus (a), and the bands from 3 separate experiments were quantified (b). *p < 0.05.

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