AB170939

重组Anti-PDE4B抗体[EPR11830]

Anti-PDE4B antibody [EPR11830]

RabMAb
Recombinant
KO Validated
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(11 Publications)

Rabbit Recombinant Monoclonal PDE4B3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 11 publications.

查看别名

DPDE4, PDE4B, PDE32, cAMP-specific phosphodiesterase 4B

3 Images

Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (AB170939)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDE4B antibody [EPR11830] (AB170939)

Intracellular flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red)at 1/10 dilution,or a rabbit IgG (negative) (green).

Lab

Western blot - Anti-PDE4B antibody [EPR11830] (AB170939)

Lanes 1-4 : Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa.

ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used. Wild-type and PDE4B knockout samples were subjected to SDS-PAGE. ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PDE4B knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PDE4B knockout HeLa cell line (<a href='/products/cell-lines/human-pde4b-knockout-hela-cell-line-ab265259'>ab265259</a>)

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Supplier Data

Western blot - Anti-PDE4B antibody [EPR11830] (AB170939)

All lanes:

Western blot - Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

Human glioma lysates at 10 µg

Lane 3:

A549 lysates at 10 µg

Predicted band size: 83 kDa

false

不同偶联物与剂型 (2)

Carrier free

Anti-PDE4B antibody [EPR11830] - BSA and Azide free
HRP

HRP Anti-PDE4B antibody [EPR11830]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11830

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4B (PDE4B) also known as PDE4B2 is an enzyme that specifically breaks down cyclic adenosine monophosphate (cAMP) to AMP an important process in cellular signaling. It belongs to the PDE4 family which is characterized by its ability to regulate the concentration of cAMP within cells. PDE4B has a molecular mass of approximately 75 kDa and is frequently expressed in immune cells including monocytes and neutrophils as well as in the brain.

Biological function summary

PDE4B plays a significant role in modulating intracellular signaling by controlling the levels of cAMP a second messenger involved in various cellular processes. As part of the larger PDE4 family complex PDE4B assists in fine-tuning immune responses and inflammatory processes. This enzyme modulates the activity of protein kinase A (PKA) impacting processes such as cell proliferation differentiation and apoptosis.

Pathways

PDE4B operates within the cAMP signaling pathway which is essential for transducing extracellular signals into cellular responses. This pathway involves interaction with other proteins such as PKA and CREB (cAMP response element-binding protein) which are critical for regulating gene expression and cellular functions. PDE4B's ability to modulate this pathway makes it fundamental in controlling the dynamics of cellular signaling.

PDE4B has been associated with inflammatory diseases such as asthma and chronic obstructive pulmonary disease (COPD). Its involvement in regulating cAMP levels affects the inflammatory response linking it to the pathogenesis of these conditions. Additionally PDE4B influences the activity of inflammatory mediators and cytokines contributing to disease progression and symptoms. For example PDE4 inhibitors can modulate these pathways presenting potential therapeutic opportunities for managing related inflammatory disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 15260978). May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.

See full target information PDE4B

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39408735

2024

Nobiletin, as a Novel PDE4B Inhibitor, Alleviates Asthma Symptoms by Activating the cAMP-PKA-CREB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Yaping Yang,Huicong Liang,Yuerun Liang,Guixin Xiong,Fang Lu,Kan Yang,Qi Zou,Xiaomin Zhang,Guanhua Du,Ximing Xu,Jiejie Hao

International journal of molecular sciences 25: PubMed39126032

2024

Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Scalavino,Emanuele Piccinno,Nicoletta Labarile,Raffaele Armentano,Gianluigi Giannelli,Grazia Serino

The international journal of neuropsychopharmacology 27: PubMed39099166

2024

Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Fangjiao Zong,Jiahao Dong,Wei Zhao,Fangfang Zhang,Wei Wang,Shuang Zhao,Ziqi Wang,Fang Zhang,Han-Ting Zhang

Cardiovascular research 120:273-285 PubMed38099489

2023

Remodelling of cAMP dynamics within the SERCA2a microdomain in heart failure with preserved ejection fraction caused by obesity and type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Lai,Susanne S Hille,Hariharan Subramanian,Robert Wiegmann,Pia Roser,Oliver J Müller,Viacheslav O Nikolaev,Kirstie A De Jong

Journal of neuroinflammation 20:187 PubMed37580715

2023

Exacerbating effects of single-dose acute ethanol exposure on neuroinflammation and amelioration by GPR110 (ADGRF1) activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sharmistha Banerjee,Taeyeop Park,Yoo Sun Kim,Hee-Yong Kim

Cell reports 42:112375 PubMed37043347

2023

Circadian protein TIMELESS regulates synaptic function and memory by modulating cAMP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Estibaliz Barrio-Alonso,Pablo J Lituma,Michael J Notaras,Robert Albero,Youcef Bouchekioua,Natalie Wayland,Isidora N Stankovic,Tanya Jain,Sijia Gao,Diany Paola Calderon,Pablo E Castillo,Dilek Colak

Cells 11: PubMed36291124

2022

Compartmentation of cGMP Signaling in Induced Pluripotent Stem Cell Derived Cardiomyocytes during Prolonged Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Faleeva,Ivan Diakonov,Prashant Srivastava,Masoud Ramuz,Gaia Calamera,Kjetil Wessel Andressen,Nadja Bork,Lorenza Tsansizi,Marie-Victoire Cosson,Andreia Sofia Bernardo,Viacheslav Nikolaev,Julia Gorelik

Frontiers in pharmacology 12:722040 PubMed34819854

2021

Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Lihua Gao,Chunsheng Shao,Mingsi Deng,Liangjian Chen

Experimental and therapeutic medicine 22:1398 PubMed34650646

2021

Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Zhang,Meifeng Liang,Xiongfei Wan

International journal of molecular medicine 44:949-959 PubMed31257455

2019

Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide‑induced pulmonary inflammation by inhibiting interleukin‑8 production and NF‑κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Won Lee,Hyung Won Ryu,Su Ui Lee,Min-Gu Kim,Ok-Kyoung Kwon,Mun Ok Kim,Tae Kyu Oh,Jae Kyoung Lee,Tae Young Kim,Sang Woo Lee,Sangho Choi,Wan-Yi Li,Kyung-Seop Ahn,Sei-Ryang Oh

View all publications

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重组Anti-PDE4B抗体[EPR11830]