Anti-PDE4A抗体(ab42094)
Key features and details
- Rabbit polyclonal to PDE4A
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-PDE4A抗体
参阅全部 PDE4A 一抗 -
描述
兔多克隆抗体to PDE4A -
宿主
Rabbit -
特异性
PDE4A5 variant specific. Does not cross-react with PDE4A8 or other PDE4A proteins including PDE4A4, PDE4A1 and PDE4Ax or with other PDE family members. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Synthetic peptide corresponding to Rat PDE4A5 (N terminal). EAW74861.1 Modified to achieve desired antigenicity and linker for covalent coupling to blue carrier protein.
Database link: P54748 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Antibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42094于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/750 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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| 说明 |
|---|
|
WB
1/750 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
靶标
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功能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
组织特异性
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). -
通路
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
翻译后修饰
Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3. -
细胞定位
Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes. - Information by UniProt
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别名
- 5''-cyclic phosphodiesterase 4A antibody
- cAMP specific phosphodiesterase antibody
- cAMP-specific 3'' antibody
see all
图片
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Western blot - Anti-PDE4A antibody (ab42094)Anti-PDE4A antibody (ab42094) + PDE4A5
Predicted band size: 108 kDa
Observed band size: 108 kDa
数据表及文件
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Datasheet download
文献 (3)
ab42094 被引用在 3 文献中.
- Katrancha SM et al. Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits. Cell Rep 26:2805-2817.e9 (2019). PubMed: 30840899
- Wilson NM et al. Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. Front Syst Neurosci 10:5 (2016). WB . PubMed: 26903822
- Oliva AA et al. Phosphodiesterase isoform-specific expression induced by traumatic brain injury. J Neurochem 123:1019-29 (2012). PubMed: 23057870
