AB200383

Anti-PDE4A抗体

Name: PDE4A抗体 (ab200383)| Abcam中文官网
Brand: Abcam Limited
SKU: AB200383
Price: 4744 CNY
Availability: InStock

Anti-PDE4A antibody

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(5 Publications)

Rabbit Polyclonal PDE4A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4A.

查看别名

DPDE2, PDE4A, PDE46, cAMP-specific phosphodiesterase 4A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4A antibody (AB200383)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4A antibody (AB200383)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PDE4A with ab200383 at 1/40 dilution.

Supplier Data

Western blot - Anti-PDE4A antibody (AB200383)

6% SDS-PAGE.

All lanes:

Western blot - Anti-PDE4A antibody (ab200383) at 1/300 dilution

Lane 1:

HeLa cell lysate at 40 µg

Lane 2:

A172 cell lysate at 40 µg

Predicted band size: 98 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human PDE4A. The exact immunogen used to generate this antibody is proprietary information.

P27815

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PDE4A or phosphodiesterase 4A is an isoform part of the PDE4 family known for hydrolyzing cAMP an important second messenger in cells. The mass of PDE4A can vary depending on the isoform with commonly referenced masses around 70-100 kDa. This enzyme is expressed in various tissues including the brain immune cells and lungs. Other popular names for this protein include PDE46 and DPDE2 indicating its different isoforms and specific functionalities across tissue types.

Biological function summary

PDE4A plays an important role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) a critical component in signaling pathways that affect numerous cellular processes. By controlling cAMP concentrations PDE4A regulates inflammatory responses and airway smooth muscle contraction which is important for respiratory functions. PDE4A does not function within a complex but operates as a homodimer when exerting its effects on signaling cascades.

Pathways

PDE4A's role is significant in the cAMP-dependent signaling pathway. This pathway influences processes like inflammation and smooth muscle relaxation. PDE4A interacts with various proteins such as PKA (protein kinase A) which is activated by cAMP and mediates several downstream effects. By influencing the cAMP pathway PDE4A affects bodily responses including immune and respiratory systems.

PDE4A has implications in conditions such as asthma and chronic obstructive pulmonary disease (COPD). Dysregulation of cAMP through PDE4A can contribute to these respiratory conditions by affecting airway inflammation and muscle tone. PDE4A is also linked to other related proteins such as PDE4B which can also impact inflammatory pathways in similar disease contexts. PDE4 inhibitors have been explored as therapeutic agents for modifying these diseases by targeting the enzymatic activity of PDE4A and related isoforms to restore balanced cAMP levels.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes.. Isoform 1. Efficiently hydrolyzes cAMP.. Isoform 2. Efficiently hydrolyzes cAMP.. Isoform 3. Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP.. Isoform 4. Efficiently hydrolyzes cAMP.. Isoform 6. Efficiently hydrolyzes cAMP.. Isoform 7. Efficiently hydrolyzes cAMP.

See full target information PDE4A

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

The international journal of neuropsychopharmacology 27: PubMed39099166

2024

Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Fangjiao Zong,Jiahao Dong,Wei Zhao,Fangfang Zhang,Wei Wang,Shuang Zhao,Ziqi Wang,Fang Zhang,Han-Ting Zhang

JCI insight 8: PubMed37427586

2023

Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Hyeon Jeong,Greg Urquhart,Cheryl Lewis,Zhikai Chi,Jenna L Jewell

Neurochemical research 47:1707-1720 PubMed35229272

2022

CircAXL Knockdown Alleviates Aβ-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shengxi Meng,Bing Wang,Wentao Li

Journal of cellular and molecular medicine 25:3460-3468 PubMed33713531

2021

H S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Jing Wang,Tao Shen,Renyun Hong,Shanshan Tang,Xia Zhao

PLoS biology 16:e2004408 PubMed29927970

2018

CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

Applications

Unspecified application

Species

Unspecified reactive species

Niloofar Ale-Agha,Christine Goy,Philipp Jakobs,Ioakim Spyridopoulos,Stefanie Gonnissen,Nadine Dyballa-Rukes,Karin Aufenvenne,Florian von Ameln,Mark Zurek,Tim Spannbrucker,Olaf Eckermann,Sascha Jakob,Simone Gorressen,Marcel Abrams,Maria Grandoch,Jens W Fischer,Karl Köhrer,René Deenen,Klaus Unfried,Joachim Altschmied,Judith Haendeler

View all publications

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Anti-PDE4A抗体

Anti-PDE4A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4A antibody (AB200383)

Western blot - Anti-PDE4A antibody (AB200383)