AB248930

重组Anti-PDE2A抗体[EPR9688] - BSA and Azide free

Anti-PDE2A antibody [EPR9688] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PDE2A antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

Cyclic GMP-stimulated phosphodiesterase, CGS-PDE, cGSPDE, PDE2A

3 Images

Unknown

Western blot - Anti-PDE2A antibody [EPR9688] - BSA and Azide free (AB248930)

This data was developed using ab140650, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PDE2A antibody [EPR9688] (<a href='/products/primary-antibodies/pde2a-antibody-epr9688-ab140650'>ab140650</a>) at 1/1000 dilution

Lane 1:

Fetal brain cell lysates at 10 µg

Lane 2:

Fetal forebrain cell lysates at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 106 kDa

false

Supplier Data

Western blot - Anti-PDE2A antibody [EPR9688] - BSA and Azide free (AB248930)

This data was developed using ab140650, the same antibody clone in a different buffer formulation.

Negative control : mouse heart, mouse spleen.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution

Dilution and blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PDE2A antibody [EPR9688] (<a href='/products/primary-antibodies/pde2a-antibody-epr9688-ab140650'>ab140650</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 105 kDa

false

Supplier Data

Western blot - Anti-PDE2A antibody [EPR9688] - BSA and Azide free (AB248930)

This data was developed using ab140650, the same antibody clone in a different buffer formulation.

Negative control : rat heart, rat spleen.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution

Dilution and blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PDE2A antibody [EPR9688] (<a href='/products/primary-antibodies/pde2a-antibody-epr9688-ab140650'>ab140650</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 105 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Unconjugated

Anti-PDE2A antibody [EPR9688]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9688

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab248930 is the carrier-free version of ab140650.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PDE2A or phosphodiesterase 2A is an enzyme involved in the breakdown of cyclic nucleotides specifically cAMP and cGMP through its phosphodiesterase activity. This protein has a mass of approximately 105 kDa and is also known as cGMP-stimulated phosphodiesterase. PDE2A is expressed in various tissues with the highest levels found in the brain heart and adrenal gland. It is essential in regulating intracellular signals making it an important player in cellular communication.

Biological function summary

PDE2A plays a significant role in modulating signaling pathways by controlling the levels of cyclic nucleotides. It does not form part of a larger complex but interacts with other proteins to exert its function. Its activity impacts processes such as smooth muscle contraction neurotransmission and cardiac function. By hydrolyzing cAMP and cGMP PDE2A influences the cellular response to external stimuli and maintains homeostasis within cells.

Pathways

Phosphodiesterase 2A significantly contributes to the cyclic nucleotide signaling pathway and the MAPK signaling pathway. By regulating cAMP and cGMP levels PDE2A influences signal transduction mechanisms linked to these pathways. It interfaces with proteins such as protein kinase A (PKA) and guanylyl cyclase affecting downstream responses that involve cell growth differentiation and apoptosis.

Abnormal PDE2A activity has implications in neurological and cardiovascular conditions. Dysregulation of PDE2A has been associated with depressive disorders and heart failure. Its relationship with other proteins such as PKA can alter neurotransmitter release in depression and affect cardiac muscle contractility in heart failure. These connections highlight PDE2A as a potential therapeutic target for managing such conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

cGMP-activated cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 15938621, PubMed : 29392776, PubMed : 9210593). Has a higher efficiency with cGMP compared to cAMP (PubMed : 15938621). Plays a role in cell growth and migration (PubMed : 24705027).. Isoform PDE2A2. Regulates mitochondrial cAMP levels and respiration (By similarity). Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L (PubMed : 28463107).

See full target information PDE2A

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PDE2A抗体[EPR9688] - BSA and Azide free