Anti-PDE10A抗体[OTI3G9] (ab114025)
![Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)](https://www.abcam.cn/ps/products/114/ab114025/Images/ab114025-126831-anti-pde10a-antibody-oti3g9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)")
Key features and details
- Mouse monoclonal [OTI3G9] to PDE10A
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-PDE10A抗体[OTI3G9]
参阅全部 PDE10A 一抗 -
描述
小鼠单克隆抗体[OTI3G9] to PDE10A -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human PDE10A aa 1-779. Produced in HEK-293T cells (NP_006652).
Database link: Q9Y233 -
阳性对照
- ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PDE10A cDNA. Flow Cyt (Intra): HEK-293T cells transfected with PDE10A overexpress plasmid.
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常规说明
Clone OTI3G9 (formerly 3G9).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI3G9 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114025于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100. |
靶标
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功能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. -
组织特异性
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. -
通路
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
结构域
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. -
细胞定位
Cytoplasm. Located mostly to soluble cellular fractions. - Information by UniProt
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数据库链接
- Entrez Gene: 10846 Human
- Omim: 610652 Human
- SwissProt: Q9Y233 Human
- Unigene: 348762 Human
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别名
- 5''-cyclic phosphodiesterase 10A antibody
- cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
- cAMP and cAMP-inhibited cGMP 3'' antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody [OTI3G9] (ab114025)
pCMV6-ENTRY PDE10A cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for PDE10A using ab114025 at a 1/100 dilution in ICC/IF.
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![Flow Cytometry (Intracellular) - Anti-PDE10A antibody [OTI3G9] (ab114025)](https://www.abcam.cn/ps/products/114/ab114025/Images/ab114025-126830-anti-pde10a-antibody-oti3g9-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PDE10A antibody [OTI3G9] (ab114025)HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PDE10A (Red) or empty vector control plasmid (Blue) were immunostained with ab114025 at 1/100 and then analyzed by flow cytometry.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab114025 尚未被引用在任何文献中。