AB151454

Anti-PDE10A 抗体 - C-terminal

Name: PDE10A抗体- C-terminal (ab151454)| Abcam中文官网
Brand: Abcam Limited
SKU: AB151454
Price: 4922 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-PDE10A antibody - C-terminal

(1 Review)

(3 Publications)

Rabbit Polyclonal PDE10A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDE10A aa 500 to C-terminus.

查看别名

PDE10A

3 Images

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)

Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)

Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.

Supplier Data

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PDE10A aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y233

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 10A (PDE10A) also known as phosphodiesterase 10 is an enzyme that hydrolyzes cyclic nucleotides such as cAMP and cGMP into non-cyclic forms which reduces their signaling effects. PDE10A has a molecular weight of approximately 89 kDa. This enzyme shows higher expression in the brain especially in the striatum a region important for motor control and certain cognitive functions. It occurs in other tissues at lower levels but its role there is less clear.

Biological function summary

PDE10A modulates intracellular signaling pathways critical for neurological processes by controlling levels of cyclic nucleotides. Although PDE10A acts independently it interacts with various signaling molecules affecting neuronal signaling. It exerts significant influence over the dopamine and glutamate neurotransmitter systems important for regulating neuronal excitability and synaptic transmission.

Pathways

Several neurological pathways rely on the function of PDE10A to maintain homeostasis. The enzyme plays a role in the cAMP and cGMP signaling pathways both of which are fundamental in mediating various cellular responses. PDE10A shares functional relationships with other phosphodiesterase family members such as PDE1 and PDE2 which also regulate cyclic nucleotide levels in the central nervous system.

An altered PDE10A function links to several neurological conditions including Huntington's disease and schizophrenia. Dysregulation of dopamine signaling within these disorders implicates PDE10A since it modulates this neurotransmitter pathway. In schizophrenia abnormal PDE10A activity could affect proteins like D2 dopamine receptors contributing to altered signaling in neural circuits associated with this disorder.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides (PubMed : 10373451, PubMed : 10393245, PubMed : 16330539, PubMed : 17389385, PubMed : 27058447). Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate (PubMed : 10373451, PubMed : 10393245, PubMed : 17389385, PubMed : 27058447). May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition (PubMed : 27058447).

See full target information PDE10A

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 148:1588-1603 PubMed39546628

2024

Single-nucleus transcriptomics reveals disease- and pathology-specific signatures in α-synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Gonzalo S Nido,Martina Castelli,Sepideh Mostafavi,Anna Rubiolo,Omnia Shadad,Guido Alves,Ole-Bjørn Tysnes,Irene H Flønes,Christian Dölle,Charalampos Tzoulis

The Prostate 82:97-106 PubMed34633095

2021

AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Ikeuchi,Yuriko Wakita,Guoxiang Zhang,Chun Li,Keiichi Itakura,Takahiro Yamakawa

Molecular therapy : the journal of the American Society of Gene Therapy 29:1086-1101 PubMed33221436

2020

PDE10A Inhibition Reduces the Manifestation of Pathology in DMD Zebrafish and Represses the Genetic Modifier PITPNA.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias R Lambert,Janelle M Spinazzola,Jeffrey J Widrick,Anna Pakula,James R Conner,Janice E Chin,Jane M Owens,Louis M Kunkel

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDE10A 抗体 - C-terminal

Anti-PDE10A antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise

Anti-PDE10A 抗体 - C-terminal

Anti-PDE10A antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise