重组Anti-PDCD7抗体[EPR8231] (ab131258)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8231] to PDCD7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PDCD7抗体[EPR8231]
参阅全部 PDCD7 一抗 -
描述
兔单克隆抗体[EPR8231] to PDCD7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.
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阳性对照
- HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 8.10 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8231 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Promotes apoptosis when overexpressed. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10081 Human
- Entrez Gene: 50996 Mouse
- Entrez Gene: 363082 Rat
- Omim: 608138 Human
- SwissProt: Q8N8D1 Human
- SwissProt: Q9WTY1 Mouse
- Unigene: 458596 Human
- Unigene: 29193 Mouse
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别名
- Apoptosis-related protein ES18 antibody
- ES18 antibody
- HES18 antibody
see all
图片
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Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
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All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)
Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131258 被引用在 1 文献中.
- Ishihara T et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013). PubMed: 23740936