重组Anti-PDCD6/ALG-2抗体[EPR3260] (ab133326)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3260] to PDCD6/ALG-2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PDCD6/ALG-2抗体[EPR3260]
参阅全部 PDCD6/ALG-2 一抗 -
描述
兔单克隆抗体[EPR3260] to PDCD6/ALG-2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Jurkat, and HepG2 whole cell lysate (ab7900).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3260 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133326于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
靶标
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功能
Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5. -
序列相似性
Contains 5 EF-hand domains. -
细胞定位
Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules. - Information by UniProt
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数据库链接
- Entrez Gene: 10016 Human
- Entrez Gene: 18570 Mouse
- Entrez Gene: 308061 Rat
- Omim: 601057 Human
- SwissProt: O75340 Human
- SwissProt: P12815 Mouse
- Unigene: 50823 Human
- Unigene: 24254 Mouse
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别名
- AIP1 antibody
- ALG 2 antibody
- ALG-2-interacting protein 1 antibody
see all
图片
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All lanes : Anti-PDCD6/ALG-2 antibody [EPR3260] (ab133326) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab133326 被引用在 7 文献中.
- Akopian D et al. Co-adaptor driven assembly of a CUL3 E3 ligase complex. Mol Cell 82:585-597.e11 (2022). PubMed: 35120648
- Claude-Taupin A et al. ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat Cell Biol 23:846-858 (2021). PubMed: 34257406
- Sønder SL et al. Annexin A7 is required for ESCRT III-mediated plasma membrane repair. Sci Rep 9:6726 (2019). PubMed: 31040365
- Liu X Up-regulation of miR-20a by HPV16 E6 exerts growth-promoting effects by targeting PDCD6 in cervical carcinoma cells. Biomed Pharmacother 102:996-1002 (2018). WB . PubMed: 29710555
- Zhang D et al. ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity. Biochem Biophys Res Commun 486:300-306 (2017). PubMed: 28300556
- Yuan L et al. MiR-124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncol Lett 14:7311-7317 (2017). PubMed: 29344168
- Choi HK et al. Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response. Nat Commun 6:7390 (2015). PubMed: 26077467