Anti-PDCD4 (phospho S457)抗体(ab226849)
Key features and details
- Rabbit polyclonal to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-PDCD4 (phospho S457)抗体
参阅全部 PDCD4 一抗 -
描述
兔多克隆抗体to PDCD4 (phospho S457) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human PDCD4 (C terminal) (phospho S457). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q53EL6 -
阳性对照
- WB: NIH/3T3 treated with PDGF, PC-12, Rat2 whole cell lysate. IHC-P: Mouse spleen and rat intestine tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226849于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
组织特异性
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
序列相似性
Belongs to the PDCD4 family.
Contains 2 MI domains. -
结构域
Binds EIF4A1 via both MI domains. -
翻译后修饰
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
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数据库链接
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 1605 Mouse
- Unigene: 375091 Mouse
- Unigene: 206228 Rat
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别名
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
图片
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Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 51 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. -
Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) treated with 5 ng/ml PDGF for 24 hours whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)Immunohistochemical analysis of paraffin-embedded rat intestine tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226849 尚未被引用在任何文献中。