Anti-PDCD4抗体(ab51495)
Key features and details
- Rabbit polyclonal to PDCD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PDCD4抗体
参阅全部 PDCD4 一抗 -
描述
兔多克隆抗体to PDCD4 -
宿主
Rabbit -
特异性
ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis -
免疫原
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
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阳性对照
- WB: 293 HEK cells, Mouse N2A cell Lysate. IHC-P: Mouse colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against human Pdcd4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/300.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/300. |
靶标
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功能
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
组织特异性
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
序列相似性
Belongs to the PDCD4 family.
Contains 2 MI domains. -
结构域
Binds EIF4A1 via both MI domains. -
翻译后修饰
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
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数据库链接
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
see all -
别名
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
图片
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All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody (ab51495)ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.
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All lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution
Lane 1 : Human HEK cell Lysate at 30 ug/ml
Lane 2 : Mouse N2A cell Lysate at 30 ug/ml
Secondary
All lanes : Li-Cor IRDye®800CW-conjugated Goat anti rabbit
Predicted band size: 52 kDa
13% SDS-Page gel used, Blocking done for 1 hour at RT, Primary Incubation overnight at 4°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab51495 被引用在 39 文献中.
- Wang X et al. WWP2 ameliorates oxidative stress and inflammation in atherosclerotic mice through regulation of PDCD4/HO-1 pathway. Acta Biochim Biophys Sin (Shanghai) 54:1057-1067 (2022). PubMed: 35983977
- Guo LM et al. Anti-PD-L1 Antibody Enhances T Cell Immune Responses and Reduces Resistance of Breast Cancer Cells to Radiotherapy. Oxid Med Cell Longev 2022:5938688 (2022). PubMed: 35295718
- Hiraku Y et al. MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck. J Occup Health 63:e12282 (2021). PubMed: 34679210
- Guan K et al. Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression. Aging (Albany NY) 13:25484-25495 (2021). PubMed: 34905503
- Yao M et al. Exosomal miR-21 secreted by IL-1ß-primed-mesenchymal stem cells induces macrophage M2 polarization and ameliorates sepsis. Life Sci 264:118658 (2021). PubMed: 33115604