Key features and details
- Rabbit polyclonal to PDCD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
参阅全部 PDCD4 一抗
特异性ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.
经测试应用适用于: WB, IHC-Pmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
- WB: 293 HEK cells, Mouse N2A cell Lysate. IHC-P: Mouse colon tissue.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
功能Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
组织特异性Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
序列相似性Belongs to the PDCD4 family.
Contains 2 MI domains.
结构域Binds EIF4A1 via both MI domains.
翻译后修饰Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
- Information by UniProt
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size: 52 kDa
Observed band size: 52 kDa
ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.
All lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution
Lane 1 : Human HEK cell Lysate at 30 ug/ml
Lane 2 : Mouse N2A cell Lysate at 30 ug/ml
All lanes : Li-Cor IRDye®800CW-conjugated Goat anti rabbit
Predicted band size: 52 kDa
13% SDS-Page gel used, Blocking done for 1 hour at RT, Primary Incubation overnight at 4°C.
ab51495 被引用在 37 文献中.
- Yao M et al. Exosomal miR-21 secreted by IL-1ß-primed-mesenchymal stem cells induces macrophage M2 polarization and ameliorates sepsis. Life Sci 264:118658 (2021). PubMed: 33115604
- Wen C et al. Circ_0003266 sponges miR-503-5p to suppress colorectal cancer progression via regulating PDCD4 expression. BMC Cancer 21:284 (2021). PubMed: 33726686
- Zhao SF et al. Long non-coding RNA KCNQ1OT1 increases the expression of PDCD4 by targeting miR-181a-5p, contributing to cardiomyocyte apoptosis in diabetic cardiomyopathy. Acta Diabetol 58:1251-1267 (2021). PubMed: 33907874
- Hiraku Y et al. MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck. J Occup Health 63:e12282 (2021). PubMed: 34679210
- Guan K et al. Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression. Aging (Albany NY) 13:25484-25495 (2021). PubMed: 34905503