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Anti-PD-L1抗体[MIH6] - Low endotoxin，Azide free (ab80276)

Reviews (5)Q&A (2)References (13)
Flow Cytometry - Anti-PD-L1 antibody [B7-H1] - Low endotoxin, Azide free (ab80276)

    Key features and details

    • Rat monoclonal [MIH6] to PD-L1 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

    选择批间可重复性更高的重组抗体

    Product image
    Anti-PD-L1 antibody [EPR23546-160] (ab252436)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-PD-L1抗体[MIH6] - Low endotoxin，Azide free
      参阅全部 PD-L1 一抗

    • 描述

      大鼠单克隆抗体[MIH6] to PD-L1 - Low endotoxin，Azide free

    • 宿主

      Rat

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • Flow Cyt: C57BL/6 mouse splenocytes.

    • 常规说明

      Please note the manufacture has changed and this item is currently only recommended for Flow Cytometry.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab80276于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use at an assay dependent concentration.

    Use = 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood.
    说明
    Flow Cyt
    Use at an assay dependent concentration.

    Use = 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood.

    靶标

    • 功能

      Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

    • 组织特异性

      Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

    • 序列相似性

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 细胞定位

      Cell membrane and Endomembrane system.
    • Information by UniProt

    • 数据库链接

      • 别名

        • B7 H antibody
        • B7 H1 antibody
        • B7 homolog 1 antibody
        • B7-H1 antibody
        • B7H antibody
        • B7H1 antibody
        • CD 274 antibody
        • CD-274 antibody
        • CD274 antibody
        • CD274 antigen antibody
        • CD274 molecule antibody
        • MGC142294 antibody
        • MGC142296 antibody
        • OTTHUMP00000021029 antibody
        • PD L1 antibody
        • PD-L1 antibody
        • PD1L1_HUMAN antibody
        • PDCD1 ligand 1 antibody
        • PDCD1L1 antibody
        • PDCD1LG1 antibody
        • PDL 1 antibody
        • PDL1 antibody
        • Programmed cell death 1 ligand 1 antibody
        • Programmed death ligand 1 antibody
        • RGD1566211 antibody
        see all

      图片

      • Flow Cytometry - Anti-PD-L1 antibody [B7-H1] - Low endotoxin, Azide free (ab80276)
        Flow Cytometry - Anti-PD-L1 antibody [B7-H1] - Low endotoxin, Azide free (ab80276)

        C57BL/6 mouse splenocytes were stained with ab80276 (purple filled histogram) or purified rat IgG2a, κ isotype control (black dashed line open histogram), followed by anti-Rat IgG PE.

      文献 (13)

      发表研究结果有使用 ab80276？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab80276 被引用在 13 文献中.

      • Murad JP  et al. Pre-conditioning modifies the TME to enhance solid tumor CAR T cell efficacy and endogenous protective immunity. Mol Ther 29:2335-2349 (2021). PubMed: 33647456
      • Chen FF  et al. Small-molecule PD-L1 inhibitor BMS1166 abrogates the function of PD-L1 by blocking its ER export. Oncoimmunology 9:1831153 (2020). PubMed: 33110706
      • Gao H  et al. Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy. J Immunother Cancer 8:N/A (2020). PubMed: 33203663
      • Sharma NS  et al. Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy. J Clin Invest 130:451-465 (2020). PubMed: 31613799
      • Brenner E  et al. Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours. Nat Commun 11:1335 (2020). PubMed: 32165639
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (skbr3 tumor taken out nude mice)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
      Permeabilization
      No
      Specification
      skbr3 tumor taken out nude mice
      Blocking step
      Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 08 2022

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free

       Poor
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mammary tumour, spleen & Lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer
      Permeabilization
      Yes - 0.4% Triton
      Specification
      Mammary tumour, spleen & Lung
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 18 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Pancreas)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Specification
      Pancreas
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Formalin
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 22 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mouse Lung Tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      Yes - Triton
      Specification
      Mouse Lung Tissue
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Ayman Oweida

      Verified customer

      提交于 Mar 14 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Bos taurus Tissue sections (Ovary (corpus luteum) and spleen as positive contr)
      Permeabilization
      No
      Specification
      Ovary (corpus luteum) and spleen as positive contr
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 06 2016

      Question

      Searching for anti-human PDL1 or anti-human PD1 to block  ligand-receptor binding

      Read More
      Answer

      We do not currently have any antibodies that are known to inhibit/neutralize either human PD-L1 or PD1 but ab80276, also known as clone MIH6, may be the best choice, though we do not have data for human reactivity, just mouse. The paper at J Exp Med. 2003 Jul 7;198(1):63-9, PMID: 12847137, describes validation by flow cytometry and an in vivo inhibition treatment protocol.

      Reactivity with human PD-L1 is uncertain. Alignment of murine PD-L1 and the human protein returns 69% identity. We do not offer free samples and our standard guarantee does not currently apply to a test of human samples, but we offer a program called Abtrial (https://www.abcam.com/content/abtrial-program-test-risk-free) for testing this antibody for inhibition of human PD-L1.

      Read More

      Question

      Can this product be used in an inhibition assay?

      Read More
      Answer

      Thank you for your enquiry. I can confirm that this product can also be used in an inhibition assay and I have changed the FuncS (Functional assay) application to Inhib (Inhibition assay) to better reflect the product's usage. Also, I was able to find a publication that used the same clone [MIH6] in inhibition assay and have included the link below. http://www.ncbi.nlm.nih.gov/pubmed/14530338 I hope this information will be helpful to you.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com