重组Anti-PD-L1抗体[EPR20529] (ab213480)
![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-482337-Rabbit-monoclonalEPR20529111-to-CD274-PDL1-western-blot-mouse.jpg "Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20529] to PD-L1
- Suitable for: ICC/IF, IP, WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-PD-L1抗体[EPR20529]
参阅全部 PD-L1 一抗 -
描述
兔单克隆抗体[EPR20529] to PD-L1 -
宿主
Rabbit -
特异性
This antibody is not suitable in IHC-FR for mouse samples
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经测试应用
适用于: ICC/IF, IP, WBmore details
不适用于: IHC-Fr or IHC-P -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse placenta lysate, HEK mPDL1 cell lysate, whole cell lysate from RAW 264.7 cells treated with Interferon-gamma and whole cell lysate from MEF cells treated with Interferon-gamma. IP: Mouse placenta lysate. ICC/IF: RAW 264.7 cells treated with IFN-? (100 ng/ml) for 24 hours.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20529 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213480于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IP |
1/30.
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| WB | (3) |
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 33 kDa).
|
| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IP
1/30. |
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WB
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 33 kDa). |
靶标
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功能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
组织特异性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane and Endomembrane system. - Information by UniProt
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数据库链接
- Entrez Gene: 60533 Mouse
- SwissProt: Q9EP73 Mouse
- Unigene: 245363 Mouse
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别名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
图片
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Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)All lanes : Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/5000 dilution
Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW264.7 treated with 100 ng/ml IFN gamma for 48 h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis WB was performed by using YCA-R20529(BF)-111A H3L2 YR120921PJ, 1:5000 dilution. Working concentration: 0.402 μg/ml.
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-473349-anti-pdl1-antibody-epr20529-western-blot-human-mouse.jpg)
Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)This image is courtesy of an anonymous collaborator.All lanes : Anti-PD-L1 antibody [EPR20529] (ab213480) at 100 µg
Lane 1 : HEK mPDL1 cell lysate at 20 µg
Lane 2 : HEK hPDL1 cell lysate at 20 µg
Lane 3 : HEK CL7 at 20 µg
Lane 4 : RAW 264.7 Wild-type (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 100 µg
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate treated with Interferon-gamma at 100 µg
Lane 6 : MEF Wild-type (Mouse embryonic fibroblast cell line) whole cell lysate at 100 µg
Lane 7 : MEF (Mouse embryonic fibroblast cell line) whole cell lysate treated with Interferon-gamma at 100 µg
Lane 8 : MEF mPDL1 KO cell lysate at 100 µg
Lane 9 : MEF mPDL1 KO treated with Interferon-gamma at 100 µg
Predicted band size: 33 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe mAb is specific for mouse mPDL1 and does not recognise hPDL1. As expected a specific 48kDa band is observed in RAW264.7 and MEFS cell extract after Interferon-gamma treatment. No bands are observed in RAW264.7 and MEFS WT and in MEFS KO with and without Interferon-gamma treatment.
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![Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-280108-anti-pd-l1-antibody-epr20529-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)Ab213480 staining PD-L1 in RAW264.7 from Mouse macrophage cell line transformed with Abelson murine leukemia virus by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% Methanol. Samples were incubated with ab213480 at 0.4 μg/ml. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab196889) was used as counterstain at 2.5 μg/ml. DAPI used as nuclear counterstain. Confocal image showing membranous staining on RAW 264.7 cells treated with IFN-γ (100 ng/ml) for 24 hours.
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![Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-276143-anti-pd-l1-antibody-epr20529-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)PD-L1 was immunoprecipitated from 0.35 mg of Mouse placenta lysate with ab213480 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab213480 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.
Lane 1: Mouse placenta lysate, 10 µg (Input).
Lane 2: ab213480 IP in mouse placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213480 in mouse placenta lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-276146-anti-pd-l1-antibody-epr20529-western-blot.jpg)
Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/1000 dilution + Mouse placenta lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 15353579.
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![Anti-PD-L1 antibody [EPR20529] (ab213480)](https://www.abcam.cn/ps/products/213/ab213480/Images/ab213480-6-benefits-of-recombinant-antibodies.png)
Anti-PD-L1 antibody [EPR20529] (ab213480)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (71)
ab213480 被引用在 71 文献中.
- Wang SS et al. Rhamnazin Enhanced Anti-Tumor Efficacy of Anti-PD-1 Therapy for Lung Cancer in Mice through Inhibition of PD-L1 Expression. Tohoku J Exp Med 260:63-73 (2023). PubMed: 36823182
- Liu N et al. Supplementation with α-ketoglutarate improved the efficacy of anti-PD1 melanoma treatment through epigenetic modulation of PD-L1. Cell Death Dis 14:170 (2023). PubMed: 36854755
- Yi J et al. Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy. Nat Commun 14:1941 (2023). PubMed: 37024504
- Saha A et al. Cysteine depletion sensitizes prostate cancer cells to agents that enhance DNA damage and to immune checkpoint inhibition. J Exp Clin Cancer Res 42:119 (2023). PubMed: 37170264
- Liu J et al. PRMT1 mediated methylation of cGAS suppresses anti-tumor immunity. Nat Commun 14:2806 (2023). PubMed: 37193698