重组Anti-PCYOX1抗体[EPR5611] (ab124966)
![Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966)](https://www.abcam.cn/ps/products/124/ab124966/Images/ab124966-529555-AntiPCYOX1-antibody-EPR5611-ab1249663sv2.jpg "Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5611] to PCYOX1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PCYOX1抗体[EPR5611]
参阅全部 PCYOX1 一抗 -
描述
兔单克隆抗体[EPR5611] to PCYOX1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human PCYOX1 aa 50-150. The exact sequence is proprietary.
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阳性对照
- SH-SY5Y, HepG2, 293T, and HeLa cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数（KD）
KD = 1.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5611 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 57 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 57 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the prenylcysteine oxidase family. -
翻译后修饰
The protein is glycosylated at one or more potential N-glycosylation sites. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 51449 Human
- Entrez Gene: 66881 Mouse
- Entrez Gene: 246302 Rat
- Omim: 610995 Human
- SwissProt: Q9UHG3 Human
- SwissProt: Q9CQF9 Mouse
- SwissProt: Q99ML5 Rat
- Unigene: 567502 Human
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别名
- EC 1.8.3.5 antibody
- KIAA0908 antibody
- PCL1 antibody
see all
图片
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Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966)All lanes : Anti-PCYOX1 antibody [EPR5611] (ab124966) at 1/10000 dilution
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) cytoplasm lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) membrane lysate
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) nuclear lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Exposure time: 3 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966)](https://www.abcam.cn/ps/products/124/ab124966/Images/PCYOX1-Primary-antibodies-ab124966-1.jpg)
Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966)All lanes : Anti-PCYOX1 antibody [EPR5611] (ab124966) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : 293T cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] (ab124966)](https://www.abcam.cn/ps/products/124/ab124966/Images/PCYOX1-Primary-antibodies-ab124966-2.jpg)
OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] (ab124966) -
![Anti-PCYOX1 antibody [EPR5611] (ab124966)](https://www.abcam.cn/ps/products/124/ab124966/Images/ab124966-3-benefits-of-recombinant-antibodies.png)
Anti-PCYOX1 antibody [EPR5611] (ab124966)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124966 尚未被引用在任何文献中。