AB124966

重组Anti-PCYOX1抗体[EPR5611]

Anti-PCYOX1 antibody [EPR5611]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal PCYOX1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

KIAA0908, PCL1, UNQ597/PRO1183, PCYOX1, Prenylcysteine oxidase 1, Prenylcysteine lyase

3 Images

Lab

Western blot - Anti-PCYOX1 antibody [EPR5611] (AB124966)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966) at 1/10000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) cytoplasm lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) membrane lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

false

Exposure time: 3s

Unknown

Western blot - Anti-PCYOX1 antibody [EPR5611] (AB124966)

All lanes:

Western blot - Anti-PCYOX1 antibody [EPR5611] (ab124966) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Lane 4:

HeLa cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] (AB124966)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PCYOX1 antibody [EPR5611] (AB124966)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PCYOX1 antibody [EPR5611] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5611

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PCYOX1 also known as prenylcysteine oxidase 1 functions as an oxidase enzyme that specifically processes prenylated proteins by cleaving the thioether bond of prenylcysteines. This protein has a molecular weight of approximately 64 kDa. Expression of PCYOX1 is noted across various tissue types with marked presence in liver and kidney. Its localization suggests a possible involvement in cellular metabolism associated with lipid-modified proteins.

Biological function summary

The PCYOX1 protein enables degradation of prenylcysteine derivatives facilitating cellular processes related to lipid anchoring of signaling molecules. It is part of a larger complex responsible for prenylated protein turnover contributing to cellular regulation by modulating levels of signal transduction proteins anchored to membranes through prenylation.

Pathways

PCYOX1 participates in the isoprenoid metabolic pathway and is intricately linked to the post-translational modification pathway. This positions it alongside other prenylation-dependent proteins such as farnesylated and geranylgeranylated proteins which rely on similar modifications to localize to their functional membrane-bound states.

Alterations in PCYOX1 activity have connections to conditions like cancer and cardiovascular diseases. Dysregulation affects protein prenylation levels impacting pathways tied to oncogenes and proteins like Ras which is often implicated in malignancies. Likewise abnormal lipid metabolism associated with PCYOX1 links to cholesterol-related cardiovascular diseases influenced by protein interactions within these pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine (PubMed : 10585463, PubMed : 11078725, PubMed : 12186880). Only active against free prenylcysteines and not prenylcysteine residues within prenylated proteins or peptides (By similarity). Involved in the final step in the degradation of prenylated proteins, by degrading prenylcysteines after the protein has been degraded (PubMed : 10585463).

See full target information PCYOX1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1645577 PubMed41048495

2025

Preliminary exploration of the ability of HUC-MSCs to restore the lung microbiota and related metabolite disorders in IPF treatment: combining 16S sequencing and metabolite analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Zhou,Yukai Luo,Jun Liu,Jishui Huang,Xiaojing Zhang,Qing-Chun Jia,Yijian Lin,Zhenyong Huang,Yiming Zeng,Wenzhao Cheng

Journal of cellular and molecular medicine 28:e70293 PubMed39720896

2024

Single-Cell RNA-Seq Reveals Injuries in Aortic Dissection and Identifies PDGF Signalling Pathway as a Potential Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Yichi Han,Yongji Cui,Juli Liu,Dingchen Wang,Guoxiang Zou,Xin Qi,Jinxiu Meng,Xiaoran Huang,Haiwei He,Xin Li

View all publications

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重组Anti-PCYOX1抗体[EPR5611]