AB181208

重组Anti-PCTAIRE1抗体[EPR13210]

Anti-PCTAIRE1 antibody [EPR13210]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal PCTAIRE1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 3 publications.

查看别名

PCTAIRE1, PCTK1, CDK16, Cyclin-dependent kinase 16, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1

4 Images

Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Intracellular flow cytometric analysis of BxPC-3cells (2% paraformaldehyde-fixed) labeling PCTAIRE1with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

Supplier Data

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

All lanes:

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

All lanes:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

All lanes:

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

Lane 1:

Human testis cell lysate at 10 µg

Lane 2:

Human fetal heart cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 56 kDa

false

Lab

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Western blot : Rabbit Monoclonal [EPR13210] to PCTAIRE1 ab181208 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in CDK16 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CDK16 knockout A549 at 20 µg

Lane 3:

HT-1080 at 20 µg

Lane 4:

HL-60 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13210

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PCTAIRE1 also known as Cyclin-dependent kinase 16 (CDK16) plays a significant mechanical role as a serine/threonine kinase. This protein with a molecular weight of approximately 45 kDa participates in various intracellular processes. It shows expression in multiple tissues including prominently in the brain and testis. PCTAIRE1 regulates cellular events by phosphorylating target proteins thereby influencing their function.

Biological function summary

PCTAIRE1 influences certain cellular functions such as signal transduction and neuron differentiation. It exists as part of complexes that include cyclins which modulate its kinase activity. In neurons PCTAIRE1 contributes to processes like neurite outgrowth and synaptic function. Research indicates a role in the transport of synaptic vesicles hinting at its involvement in neurotransmitter release and communication between neurons.

Pathways

PCTAIRE1 serves as a component of pathways such as the Wnt signaling pathway and the cell cycle. Its function aligns with several other proteins including CDK16 in modulating processes like cell proliferation and neural development. PCTAIRE1 interacts closely with proteins like Cyclin Y which help guide its role in these pathways ensuring proper progression of related biological events.

PCTAIRE1 has associations with neurological disorders and certain cancers. Abnormal expression or activity of PCTAIRE1 may relate to conditions like schizophrenia or glioblastoma. The protein's interaction with Cyclin Y is key in the context of cancer where dysregulation of these interactions can contribute to tumor progression. Alterations in PCTAIRE1 function and its pathways highlight its potential as a target for therapeutic strategies in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).

See full target information CDK16

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Movement disorders : official journal of the Movement Disorder Society 39:2291-2297 PubMed39314081

2024

Sacsin levels in PBMCs: A diagnostic assay for SACS variants in peripheral blood cells - A PROSPAX study.

Applications

Unspecified application

Species

Unspecified reactive species

Ceren Tunca,Eylül Ece İşlek Camadan,Natalia Smolina,Robin J Palvadeau,Özgür Öztop Çakmak,Atay Vural,Andreas Traschütz,Filippo M Santorelli,Bernard Brais,Rebecca Schüle,Matthis Synofzik,A Nazlı Başak

World journal of gastrointestinal surgery 16:2003-2011 PubMed39087106

2024

Diagnostic significance of serum levels of serum amyloid A, procalcitonin, and high-mobility group box 1 in identifying necrotising enterocolitis in newborns.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Guo,Zhi-Hui Jiang,Hong-Zhen Liu,Lei Zhang

Experimental and therapeutic medicine 22:843 PubMed34149889

2021

Long non-coding RNA TPT1-AS1 alleviates cell injury and promotes the production of extracellular matrix by targeting the microRNA-324-5p/CDK16 axis in human dermal fibroblasts after thermal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Luo,Haoyuan You,Jianhua Zhan,Guanghua Guo,Xing Cheng,Guoyu Zheng

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PCTAIRE1抗体[EPR13210]

Anti-PCTAIRE1 antibody [EPR13210]

Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

Western blot - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原