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AB238995

重组Anti-PCSK9抗体[EPR7627(2)] - BSA and Azide free

Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PCSK9 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

查看别名

NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9

3 Images
Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • WB

Supplier Data

Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab181142).

All lanes:

Western blot - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>) at 1/10000 dilution

Lane 1:

A431 (Human epidermoid carcinoma cell line) cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 62 kDa,78 kDa

false

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • IP

Lab

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.

Lane 1 (input) : HepG2 whole cell lysate (10µg)

Lane 2 (+) : ab181142 + HepG2 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

All lanes:

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>)

Predicted band size: 74 kDa

Observed band size: 62 kDa,78 kDa

false

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • IP

Supplier Data

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

All lanes:

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>)

Predicted band size: 74 kDa

Observed band size: 62 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7627(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information PCSK9
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