Anti-PCSK9抗体(ab31762)
Key features and details
- Rabbit polyclonal to PCSK9
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PCSK9抗体
参阅全部 PCSK9 一抗 -
描述
兔多克隆抗体to PCSK9 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IP, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse PCSK9 aa 650 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32727) -
阳性对照
- This antibody gave a positive signal in the following tissue lysates: Liver (Mouse) Kidney (Mouse) Liver (Rat) Kidney (Rat) This antibody gave a positive signal in the following cell types: PC12. It also gave a positive signal in FFPE mouse large intestine tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31762于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 74 kDa).
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| IP |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 74 kDa). |
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IP
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
组织特异性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
疾病相关
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
序列相似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻译后修饰
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 100102 Mouse
- Entrez Gene: 298296 Rat
- SwissProt: Q80W65 Mouse
- SwissProt: P59996 Rat
- Unigene: 133268 Mouse
- Unigene: 19195 Rat
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别名
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
see all
图片
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Western blot - Anti-PCSK9 antibody (ab31762)All lanes : Anti-PCSK9 antibody (ab31762) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
ab31762 detects a band of ~62 kDa in liver and kidney lysates from mouse and rat. This corresponds to the cleaved form of PCSK9. ab31762 has not detected the 74 kDa proprotein form in the lysates tested. -
Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody (ab31762)ICC/IF image of ab31762 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31762 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK9 antibody (ab31762)IHC image of ab31762 staining in mouse large intestine formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31762, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunoprecipitation - Anti-PCSK9 antibody (ab31762)PCSK9 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5µg of Rabbit polyclonal to PCSK9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31762.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 62kDa; PCSK9
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab31762 被引用在 20 文献中.
- Guo BC et al. Di-(2-ethylhexyl) Phthalate Limits the Lipid-Lowering Effects of Simvastatin by Promoting Protein Degradation of Low-Density Lipoprotein Receptor: Role of PPARγ-PCSK9 and LXRα-IDOL Signaling Pathways. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830035
- Wang Y et al. Dendritic cell-derived exosomal miR-3064-5p inhibits SIRT6/PCSK9 to protect the blood-brain barrier after subarachnoid hemorrhage. J Biochem Mol Toxicol 37:e23346 (2023). PubMed: 36988443
- Feng Z et al. PCSK9 causes inflammation and cGAS/STING pathway activation in diabetic nephropathy. FASEB J 37:e23127 (2023). PubMed: 37561547
- Fedotkina O et al. Neuronal Dysfunction Is Linked to the Famine-Associated Risk of Proliferative Retinopathy in Patients With Type 2 Diabetes. Front Neurosci 16:858049 (2022). PubMed: 35600617
- Sun Y et al. S-palmitoylation of PCSK9 induces sorafenib resistance in liver cancer by activating the PI3K/AKT pathway. Cell Rep 40:111194 (2022). PubMed: 35977495