Anti-PCSK9抗体(ab28770)
Key features and details
- Goat polyclonal to PCSK9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PCSK9抗体
参阅全部 PCSK9 一抗 -
描述
山羊多克隆抗体to PCSK9 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
CRSRHLAQASQELQ
, corresponding to C terminal amino acids 679-692 of Human PCSK9 -
阳性对照
- WB: Human adipose lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab28770 was purified from goat serum by ammonium sulphate precipitation prior to immunogen affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 74 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 74 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
组织特异性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
疾病相关
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
序列相似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻译后修饰
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
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别名
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
see all
图片
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Western blot - Anti-PCSK9 antibody (ab28770)Anti-PCSK9 antibody (ab28770) at 0.3 µg/ml + Human Adipose lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab28770 被引用在 15 文献中.
- D'Onofrio N et al. SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells. Theranostics 13:531-542 (2023). PubMed: 36632236
- Wang L et al. PCSK9 promotes the progression and metastasis of colon cancer cells through regulation of EMT and PI3K/AKT signaling in tumor cells and phenotypic polarization of macrophages. J Exp Clin Cancer Res 41:303 (2022). PubMed: 36242053
- Ramin-Mangata S et al. Effects of proprotein convertase subtilisin kexin type 9 modulation in human pancreatic beta cells function. Atherosclerosis 326:47-55 (2021). PubMed: 33933263
- Hong J et al. The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion. Foods 10:N/A (2021). PubMed: 34829135
- Dong J et al. microRNA-483 ameliorates hypercholesterolemia by inhibiting PCSK9 production. JCI Insight 5:N/A (2020). PubMed: 33119548