Anti-PCSK9抗体(ab272907)
Key features and details
- Rabbit polyclonal to PCSK9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PCSK9抗体
参阅全部 PCSK9 一抗 -
描述
兔多克隆抗体to PCSK9 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human PCSK9. The exact sequence is proprietary. Center region.
Database link: Q8NBP7 -
阳性对照
- WB: HeLa, A431 and HepG2 whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
靶标
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功能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
组织特异性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
疾病相关
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
序列相似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻译后修饰
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
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别名
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
see all
图片
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Western blot - Anti-PCSK9 antibody (ab272907)All lanes : Anti-PCSK9 antibody (ab272907) at 1/500 dilution
Lane 1 : HeLa Wild type cell lysate
Lane 2 : HeLa PCSK9 knockout cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Western blot - Anti-PCSK9 antibody (ab272907)All lanes : Anti-PCSK9 antibody (ab272907) at 1/500 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272907 尚未被引用在任何文献中。