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Cell Biology Cell Cycle Markers

Anti-PCNA抗体[PC10] (ab29)

  • Datasheet
  • SDS
Reviews (70)Q&A (34)References (517)

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Western blot - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Western blot - Anti-PCNA antibody [PC10] (ab29)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab29)
  • Flow Cytometry - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)

Key features and details

  • Mouse monoclonal [PC10] to PCNA
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-PCNA抗体[PC10]
    参阅全部 PCNA 一抗
  • 描述

    小鼠单克隆抗体[PC10] to PCNA
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pigeon, Pig, Drosophila melanogaster, Monkey, Zebrafish, Thornback ray, Dogfish, Catshark
  • 免疫原

    Fusion protein corresponding to PCNA. Protein A-PCNA fusion protein obtained from pC2T construct. This construct lacked 93 nucleotides at the 3' end of PCNA.

  • 阳性对照

    • WB: HeLa, HEK293, A431 whole cell lysates, PC12, SV40LT-SMC, NIH 3T3, rat liver, rat heart. IHC-P: Human breast (Paget's disease of the nipple), mouse liver, mouse gut, rat spinal cord DRG, developing chick brain, zebrafish intestine, rat spleen, rat large intestine. IHC-Fr: mouse embryonic brain, HeLa. Flow Cyt: HeLa.
  • 常规说明

    This antibody clone [PC10] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    PC10
  • 骨髓瘤

    Sp2/0-Ag14
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Markers
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Isotype/Loading Controls
    • Loading Controls
    • PCNA

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-PCNA antibody [PC10] (ab201672)
    • HRP Anti-PCNA antibody [PC10] (ab201673)
    • Alexa Fluor® 647 Anti-PCNA antibody [PC10] (ab201674)
    • PE Anti-PCNA antibody [PC10] (ab233658)
    • Anti-PCNA antibody [PC10] - BSA and Azide free (ab264494)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human PCNA protein (ab172197)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab29于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (28)
1/10000 - 1/30000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (19)
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
ICC/IF (13)
Use a concentration of 1 - 5 µg/ml.

Methanol fixation recommended

Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

说明
IHC-P
1/10000 - 1/30000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
ICC/IF
Use a concentration of 1 - 5 µg/ml.

Methanol fixation recommended

Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • 序列相似性

    Belongs to the PCNA family.
  • 翻译后修饰

    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • 细胞定位

    Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Target information above from: UniProt accession P12004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 373984 Chicken
    • Entrez Gene: 515499 Cow
    • Entrez Gene: 37290 Drosophila melanogaster
    • Entrez Gene: 5111 Human
    • Entrez Gene: 18538 Mouse
    • Entrez Gene: 692192 Pig
    • Entrez Gene: 25737 Rat
    • Entrez Gene: 30678 Zebrafish
    • Omim: 176740 Human
    • SwissProt: Q9DEA3 Chicken
    • SwissProt: Q3ZBW4 Cow
    • SwissProt: P17917 Drosophila melanogaster
    • SwissProt: P12004 Human
    • SwissProt: P17918 Mouse
    • SwissProt: P04961 Rat
    • SwissProt: Q9PTP1 Zebrafish
    • Unigene: 4751 Drosophila melanogaster
    • Unigene: 147433 Human
    • Unigene: 728886 Human
    • Unigene: 7141 Mouse
    • Unigene: 223 Rat
    • Unigene: 35605 Zebrafish
    see all
  • 别名

    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

图片

  • Western blot - Anti-PCNA antibody [PC10] (ab29)
    Western blot - Anti-PCNA antibody [PC10] (ab29)
    All lanes : Anti-PCNA antibody [PC10] (ab29) at 1 µg/ml

    Lane 1 : HeLa 20 ug
    Lane 2 : PC12 20 ug
    Lane 3 : SV40LT-SMC 20 ug
    Lane 4 : NIH 3T3 20 ug
    Lane 5 : Rat liver 20 ug
    Lane 6 : Rat heart 20 ug

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 29 kDa



    Merged signal (red and green). Green - ab29 observed at 32 kDa. Red - loading control, ab52866, observed at 50 kDa.

    All samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab29 and ab52866(Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/mL and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on human tissue sections (Paget's disease of the nipple). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200).

    See Abreview

  • Western blot - Anti-PCNA antibody [PC10] (ab29)
    Western blot - Anti-PCNA antibody [PC10] (ab29)
    All lanes : Anti-PCNA antibody [PC10] (ab29) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 4 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab29)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab29)

    ab29 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab29 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117 (pseudo-colored red) and ab150080 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Flow Cytometry - Anti-PCNA antibody [PC10] (ab29)
    Flow Cytometry - Anti-PCNA antibody [PC10] (ab29)

    Overlay histogram showing HeLa cells stained with ab29 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
    ab29 at 1/6000 staining mouse embryo (day 17) liver and gut tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was blocked before incubation with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for PCNA antibody [PC10] - Proliferation Marker (ab29) on Rat Tissue sections (adult spinal cord DRG). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/6000 for 2 minutes at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on E6 developing chick brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: This image shows developing brain/overlying skin.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections;1/6000 for 2h at RT) on intestine of adult zebrafish). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: The crypt nuclei on this image of zebrafish intestine, are positive for the PCNA/PC10 clone conforming to accepted localisation data for PCNA in other species.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)

    IHC image of PCNA staining in rat large intestine formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29, 0.025µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)

    IHC image of PCNA staining in rat spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29, 1/30,000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)Gong, C. et al PLoS One. 2010 Dec 20;5(12):e15630. doi: 10.1371/journal.pone.0015630 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Aldehyde dehydrogenase (ALDH1) expression correlates with clinical outcome of breast cancer patients

    (A) Immunohistochemical staining shows tumors with poor clinical response (progressive or stable disease, PD/SD) to neo-adjuvant chemotherapy express high ALDH1 (>20% positive cancer cells) in pre-chemotherapy samples, and tumors with partal response (PR) express low ALDH1 (≤20% positive cancer cells). High proliferating cell nuclear antigen (PCNA)(>25% positive cancer cells) and poor apoptosis are observed in tumors with PD/SD after neo-adjuvant chemotherapy. Representative images of ALDH1 (×200), PCNA (×200) and Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP labeling (TUNEL,×400).

    PCNA is detected using ab29 at 1/100 dilution.

    (From Figure 1A of Gong et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)Crabbe et al PLoS One. 2015 May 11;10(5):e0126846. doi: 10.1371/journal.pone.0126846. eCollection 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Annexin V and PCNA staining of decellularized lung scaffolds recellularized with (A) MSCs in static versus bioreactor conditions for 14 (panels A, B for annexin V, and a, b for PCNA) and 28 days (panels C, D for annexin V, and c, d for PCNA) (single cells), (B) MSC cell clusters in static conditions at 14 days (panel A for annexin V, and a for PCNA), (C) C10 cells in static (panel A for annexin V, a for PCNA) versus bioreactor (panel B for annexin V, b for PCNA) conditions for 11 days.

    An inset in Fig 4Ab with higher magnification is shown to demonstrate that a majority of the cells stained positive for PCNA. Cell nuclei are labeled in blue; marker of interest is labeled in green. Magnifications are 400x. Overlap of cell nucleus and marker of interest can generate green or white color. For each condition, images are representative of the entire lung.

    MSCs = Bone marrow-derived mouse mesenchymal stromal (stem) cells.

    PCNA is detected using ab29 at 1/1000 dilution.

    (From Figure 4 of Crabbe et al)

     

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (517)

发表研究结果有使用 ab29?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab29 被引用在 517 文献中.

  • Liu F  et al. LncRNA LIFR-AS1 overexpression suppressed the progression of serous ovarian carcinoma. J Clin Lab Anal N/A:e25470 (2022). WB ; Human . PubMed: 35778954
  • Cheung Y  et al. Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage. J Pediatr Surg 56:352-359 (2021). PubMed: 32709532
  • Yang Y  et al. Rebalancing TGF-ß/Smad7 signaling via Compound kushen injection in hepatic stellate cells protects against liver fibrosis and hepatocarcinogenesis. Clin Transl Med 11:e410 (2021). PubMed: 34323416
  • Li L  et al. MRC2 Promotes Proliferation and Inhibits Apoptosis of Diabetic Nephropathy. Anal Cell Pathol (Amst) 2021:6619870 (2021). PubMed: 34012764
  • Bai H  et al. Application of the Tissue-Engineered Plant Scaffold as a Vascular Patch. ACS Omega 6:11595-11601 (2021). PubMed: 34056315
View all Publications for this product

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16 Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Specification
B16 Pancreas
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 31 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Panc1 Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
Permeabilization
No
Specification
Panc1 Tumor
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 29 2021

Immunohistochemistry (Frozen sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 19 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Eisenia fetida Tissue sections (Gut)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Gut
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Oct 24 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody [PC10]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 11 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK-t2)
Permeabilization
Yes - 0.5% Triton X-100 in PBS, 20min, RT
Specification
MDCK-t2
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 01 2021

Western blot abreview for Anti-PCNA antibody [PC10]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
HEPG2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

提交于 Oct 19 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

提交于 Mar 27 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody [PC10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 27 2020

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