Anti-PCK1/PEPC抗体(ab115693)
Key features and details
- Goat polyclonal to PCK1/PEPC
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PCK1/PEPC抗体
参阅全部 PCK1/PEPC 一抗 -
描述
山羊多克隆抗体to PCK1/PEPC -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human PCK1/PEPC aa 500-600 (internal sequence) (Cysteine residue).
Database link: NP_002582.3 -
阳性对照
- Human kidney and liver tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
组织特异性
Major sites of expression are liver, kidney and adipocytes. -
通路
Carbohydrate biosynthesis; gluconeogenesis. -
疾病相关
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
序列相似性
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
翻译后修饰
Acetylation is increased on addition of glucose and appears to regulate the protein stability. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 282855 Cow
- Entrez Gene: 403560 Dog
- Entrez Gene: 5105 Human
- Entrez Gene: 18534 Mouse
- Entrez Gene: 100144531 Pig
- Entrez Gene: 362282 Rat
- Entrez Gene: 379844 Xenopus laevis
- Omim: 614168 Human
see all -
别名
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody (ab115693)
ab115693, at 2µg/ml, staining PCK1//PEPC in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody (ab115693)ab115693, at 2µg/ml, staining PCK1/PEPC in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab115693 被引用在 1 文献中.
- Zhao L et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). PubMed: 31351426