AB205016

Anti-PCIF1抗体

Name: PCIF1抗体 (ab205016)| Abcam中文官网
Brand: Abcam Limited
SKU: AB205016
Price: 4606 CNY
Availability: InStock

Anti-PCIF1 antibody

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(5 Publications)

Rabbit Polyclonal PCIF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PCIF1 aa 650 to C-terminus.

查看别名

C20orf67, CAPAM, PPP1R121, PCIF1, mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase, Cap-specific adenosine methyltransferase, Phosphorylated CTD-interacting factor 1, Protein phosphatase 1 regulatory subunit 121, hCAPAM, hPCIF1

2 Images

Supplier Data

Immunoprecipitation - Anti-PCIF1 antibody (AB205016)

Detection of PCIF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205016 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab205016 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PCIF1 antibody (ab205016)

Predicted band size: 81 kDa

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Supplier Data

Western blot - Anti-PCIF1 antibody (AB205016)

All lanes:

Western blot - Anti-PCIF1 antibody (ab205016) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 81 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human PCIF1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H4Z3

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab205016 was affinity purified using an epitope specific to PCIF1 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PCIF1 also known as Phosphorylated CTD-interacting factor 1 is a protein with a mass of around 66 kDa. It functions mechanically as an enzyme that methylates the 2'-O position of RNA cap structures modulating mRNA stability and translation. You find PCIF1 expression predominantly in the nucleus of human cells where it interacts with the C-terminal domain of RNA polymerase II. Its role is essential in RNA processing impacting the regulation of gene expression at a post-transcriptional level.

Biological function summary

PCIF1 modifies RNA molecules influencing mRNA cap structure formation and methylation. This activity is a part of the cap-binding complex which ensures proper RNA maturation and function. By interacting with the cap structure it plays a non-ignorable role in the control of RNA export translation and degradation facilitating the precise regulation of genetic information flow within cells. Its presence in the methyltransferase complex highlights its importance in cell biology.

Pathways

PCIF1 plays a prominent role in the mRNA capping and processing pathways. It partners with proteins such as RNA polymerase II in the pathway to ensure successful transcription and subsequent RNA modifications. The involvement in the mRNA cap methylation pathway highlights its connection with transcription and subsequent mRNA fate marking it as an important regulator in post-transcriptional gene expression regulation.

PCIF1 has been associated with conditions such as cancer and neurodegenerative diseases. Altered expression or function of PCIF1 can disrupt normal cellular processes leading to the progression of these disorders. Studies link PCIF1 changes to irregular mRNA methylation patterns which may relate to oncogenic transformation or neuronal dysfunction. Related proteins like RNA polymerase II also play important roles in understanding these disease mechanisms as they interact within the same pathways disrupted during disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs (PubMed : 30467178, PubMed : 30487554, PubMed : 31279658, PubMed : 31279659, PubMed : 33428944). Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally (PubMed : 30467178).

See full target information PCIF1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:3234-3256 PubMed40093906

2025

CRISPR screening reveals ZNF217 as a vulnerability in high-risk B-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Qin,Keren Zhou,Lei Dong,Lu Yang,Wei Li,Zhenhua Chen,Chao Shen,Li Han,Yangchan Li,Anthony K N Chan,Sheela Pangeni Pokharel,Ying Qing,Meiling Chen,Kitty Wang,Keith Leung,Lillian Sau,Chun-Wei Chen,Xiaolan Deng,Rui Su,Jianjun Chen

Frontiers in cell and developmental biology 11:1240039 PubMed37691832

2023

Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Bilekova,Balma Garcia-Colomer,Alberto Cebrian-Serrano,Silvia Schirge,Karsten Krey,Michael Sterr,Thomas Kurth,Stefanie M Hauck,Heiko Lickert

Cell discovery 8:48 PubMed35597784

2022

mAm methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhuo,Meng Sun,Kun Wang,Lu Zhang,Kai Li,Danyang Yi,Mengjie Li,Qiang Sun,Xixi Ma,Wei Liu,Lisong Teng,Chengqi Yi,Tianhua Zhou

Open biology 10:190306 PubMed32097574

2020

CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures.

Applications

Unspecified application

Species

Unspecified reactive species

Alison Galloway,Abdelmadjid Atrih,Renata Grzela,Edward Darzynkiewicz,Michael A J Ferguson,Victoria H Cowling

Molecular cell 75:631-643.e8 PubMed31279658

2019

Identification of the mAm Methyltransferase PCIF1 Reveals the Location and Functions of mAm in the Transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantinos Boulias,Diana Toczydłowska-Socha,Ben R Hawley,Noa Liberman,Ken Takashima,Sara Zaccara,Théo Guez,Jean-Jacques Vasseur,Françoise Debart,L Aravind,Samie R Jaffrey,Eric Lieberman Greer

View all publications

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Anti-PCIF1抗体

Anti-PCIF1 antibody

Immunoprecipitation - Anti-PCIF1 antibody (AB205016)

Western blot - Anti-PCIF1 antibody (AB205016)