AB96729

Anti-PCCB 抗体

Name: PCCB抗体 (ab96729)| Abcam中文官网
Brand: Abcam Limited
SKU: AB96729
Price: 4606 CNY
Availability: InStock

Anti-PCCB antibody

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(3 Publications)

Rabbit Polyclonal PCCB antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCCB aa 150-450.

查看别名

PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling PCCB protein at mitochondria with ab96729 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Unknown

Western blot - Anti-PCCB antibody (AB96729)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PCCB antibody (ab96729) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 58 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14, using ab96729 at 1 : 100 dilution.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PCCB aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P05166

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PCCB also known as Propionyl-CoA Carboxylase β subunit is an enzyme component that plays an important role in metabolizing certain amino acids and odd-chain fatty acids. This protein partners with the alpha subunit to form a dimeric enzyme with a total mass of approximately 750 kDa. PCCB is mainly expressed in the liver but its expression also occurs in other tissues such as the kidney and heart where energy and metabolism play key roles.

Biological function summary

Propionyl-CoA Carboxylase β subunit takes part in the process of carboxylation of propionyl-CoA to methylmalonyl-CoA an essential step in catabolism. This subunit contributes to the function of the complex necessary for efficient energy production and proper detoxification of specific intermediary products. In this enzymatic action it works jointly with the alpha subunit that harbors the biotin-binding sites important for transferring carbon dioxide.

Pathways

PCCB is an important component in the propionic acid pathway and is critical for maintaining the balance in mitochondrial fatty acid synthesis. Its function plays a central role in the catabolism of certain amino acids including valine isoleucine and methionine as well as odd-chain fatty acids. Propionyl-CoA Carboxylase β subunit’s action works closely with methylmalonyl-CoA mutase assisting in metabolic conversion that takes place after the initial carboxylation step.

Deficiencies or mutations in PCCB are linked to propionic acidemia a metabolic disorder that interrupts normal propionic acid breakdown leading to an accumulation that is toxic to the body. This deficiency relates it to methylmalonic acidemia if methylmalonyl-CoA mutase is affected often causing similar clinical symptoms such as developmental delays vomiting and metabolic crises. Understanding the function and pathways of PCCB helps to develop targeted therapies that address its metabolic roles and its involvement in these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed : 15890657, PubMed : 6765947). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (PubMed : 15890657, PubMed : 6765947). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed : 6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).

See full target information PCCB

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 47:1799-1812 PubMed37434531

2023

RGMb impacts partial epithelial-mesenchymal transition and BMP2-Induced ID mRNA expression independent of PD-L2 in nonsmall cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie Rahbek Dorset,Tina Fuglsang Daugaard,Trine Vilsbøll Larsen,Anders Lade Nielsen

Orphanet journal of rare diseases 17:62 PubMed35189944

2022

Therapeutic potential of living donor liver transplantation from heterozygous carrier donors in children with propionic acidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Gui Zeng,Guang-Peng Zhou,Lin Wei,Wei Qu,Ying Liu,Yu-Le Tan,Jun Wang,Li-Ying Sun,Zhi-Jun Zhu

Thyroid : official journal of the American Thyroid Association 29:845-857 PubMed30990120

2019

Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Strickaert,Cyril Corbet,Selim-Alex Spinette,Ligia Craciun,Geneviève Dom,Guy Andry,Denis Larsimont,Ruddy Wattiez,Jacques E Dumont,Olivier Feron,Carine Maenhaut

View all publications

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Anti-PCCB 抗体

Anti-PCCB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Western blot - Anti-PCCB antibody (AB96729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)