Anti-PCB抗体[3H2AD9] (ab110314)
![Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.cn/ps/products/110/ab110314/Images/ab110314-123596-anti-pcb-antibody-3h2ad9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)")
Key features and details
- Mouse monoclonal [3H2AD9] to PCB
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-PCB抗体[3H2AD9]
参阅全部 PCB 一抗 -
描述
小鼠单克隆抗体[3H2AD9] to PCB -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, IPmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
-
阳性对照
- Human fibroblast and HeLa cells; Human liver tissue lysate.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯化说明
ab110314 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
3H2AD9 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml. For 2 hours.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. For 2 hours. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
靶标
-
功能
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. -
通路
Carbohydrate biosynthesis; gluconeogenesis. -
疾病相关
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia. -
序列相似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 5091 Human
- Omim: 608786 Human
- SwissProt: P11498 Human
- Unigene: 89890 Human
-
别名
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color. -
![Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.cn/ps/products/110/ab110314/Images/ab110314-123595-anti-pcb-antibody-3h2ad9-immunoprecipitation.gif)
Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate. -
![Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)](https://www.abcam.cn/ps/products/110/ab110314/Images/ab110314-123594-anti-pcb-antibody-3h2ad9-flow-cytometry.gif)
Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab110314 被引用在 3 文献中.
- Cheng L et al. Long noncoding RNA RP11-241J12.3 targeting pyruvate carboxylase promotes hepatocellular carcinoma aggressiveness by disrupting pyruvate metabolism and the DNA mismatch repair system. Mol Biomed 3:4 (2022). PubMed: 35122182
- Nagaraj R et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell 168:210-223.e11 (2017). PubMed: 28086092
- Lussey-Lepoutre C et al. Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nat Commun 6:8784 (2015). IF . PubMed: 26522426