Anti-PC4抗体(ab72132)
Key features and details
- Rabbit polyclonal to PC4
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PC4抗体
参阅全部 PC4 一抗 -
描述
兔多克隆抗体to PC4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residues 25 and 75 of human PC4, NP_006704.2.
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阳性对照
- Whole cell lysate from Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72132于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 18, 190 kDa (predicted molecular weight: 14 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 18, 190 kDa (predicted molecular weight: 14 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). -
序列相似性
Belongs to the transcriptional coactivator PC4 family. -
翻译后修饰
Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10923 Human
- Omim: 600503 Human
- SwissProt: P53999 Human
- Unigene: 229641 Human
- Unigene: 644972 Human
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别名
- Activated RNA polymerase II transcriptional coactivator p15 antibody
- Interferon related developmental regulator 1 antibody
- MGC102747 antibody
see all
图片
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Western blot - Anti-PC4 antibody (ab72132)All lanes : Anti-PC4 antibody (ab72132) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab72132)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PC4 with ab72132 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-PC4 antibody (ab72132)Immunoprecipitation/ Western Blot of PC4.
Lane 1: ab72132 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72132 at 0.1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab72132)IHC image of ab72132 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72132, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab72132 被引用在 3 文献中.
- Sun T et al. The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma. J Oncol 2021:9958483 (2021). PubMed: 34899911
- Brzek A et al. Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression. BMC Mol Biol 19:9 (2018). PubMed: 30053800
- Liang XH et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). PubMed: 29165591