重组Anti-PC1/3抗体[EPR21908] (ab220363)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21908] to PC1/3
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PC1/3抗体[EPR21908]
参阅全部 PC1/3 一抗 -
描述
兔单克隆抗体[EPR21908] to PC1/3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IHC-Fr, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Beta-TC-6 whole cell lysate. Mouse hypothalamus lysate. IHC-P: Human pancreas and colon tissue. Mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue. ICC/IF: Beta-TC-6 cells. Flow Cyt (intra): Beta-TC-6 cells. IP: Beta-TC-6 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220363于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
1/500.
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ICC/IF |
1/100.
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IP |
1/30.
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Flow Cyt (Intra) |
1/50.
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说明 |
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WB
1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
1/500. |
ICC/IF
1/100. |
IP
1/30. |
Flow Cyt (Intra)
1/50. |
靶标
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功能
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. -
疾病相关
Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones. -
序列相似性
Belongs to the peptidase S8 family. Furin subfamily. -
细胞定位
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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数据库链接
- Entrez Gene: 5122 Human
- Entrez Gene: 18548 Mouse
- Entrez Gene: 25204 Rat
- Omim: 162150 Human
- SwissProt: P29120 Human
- SwissProt: P63239 Mouse
- SwissProt: P28840 Rat
- Unigene: 78977 Human
see all -
别名
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
see all
图片
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All lanes : Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate
Lane 2 : Mouse pancreas lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Additional bands at: 66 kDa (possible mature (processed) protein)
Exposure time: 80 secondsBlocking and Diluting Buffer: 5% NFDM /TBST
87 kDa ProPC1/3; 66 kDa mature form (PMID: 26778167)
Lane1 Expsoure Time: 20 seconds
Lane2 and Lane3 were developed using a high sensitivity ECL substrate.
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All lanes : Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 125 secondsBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form; the 87 kDa represents the ProPC1/3 intermediate (PMID: 26778167).
This blot was developed using a higher sensitivity ECL substrate.
Negative control: NIH/3T3 (PMID: 9405066; PMID:15143067).
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Immunohistochemical analysis of frozen rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in rat pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
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Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution + Mouse hypothalamus lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 3 minutesBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
The 66 kDa band is the mature form of PC1/3 (PMID: 26778167).
This blot was developed using a higher sensitivity ECL substrate.
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Immunohistochemical analysis of frozen mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in mouse pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in rat pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining (arrows) in human colon (PMID: 18706454) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling PC1/3 with ab220363 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in Beta-TC-6 cell line.
Negative cell control: NIH/3T3 (PMID: 9405066; PMID:15143067).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cell line labeling PC1/3 with ab220363 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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PC1/3 was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab220363 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate 10 µg (Input).
Lane 2: ab220363 IP in Beta-TC-6 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220363 in Beta-TC-6 whole cell lysate (-).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form (PMID: 26778167).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab220363 被引用在 3 文献中.
- Li M et al. VAMP4 regulates insulin levels by targeting secretory granules to lysosomes. J Cell Biol 221:N/A (2022). PubMed: 36053215
- Ma L et al. Sennoside A Induces GLP-1 Secretion Through Activation of the ERK1/2 Pathway in L-Cells. Diabetes Metab Syndr Obes 13:1407-1415 (2020). PubMed: 32425572
- Kreutzberger AJB et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Elife 9:N/A (2020). PubMed: 33164744