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Anti-PC1/3抗体(ab3532)

Reviews (1)Q&A (2)References (4)
Western blot - Anti-PC1/3 antibody (ab3532)
  • Western blot - Anti-PC1/3 antibody (ab3532)

Key features and details

  • Rabbit polyclonal to PC1/3
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-PC1/3 antibody [EPR21908] (ab220363)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-PC1/3抗体
    参阅全部 PC1/3 一抗

  • 描述

    兔多克隆抗体to PC1/3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse
    预测可用于: Rat, Cow, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3532于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).
说明
WB
Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).

靶标

  • 功能

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

  • 疾病相关

    Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

  • 序列相似性

    Belongs to the peptidase S8 family. Furin subfamily.

  • 细胞定位

    Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • BDP antibody
    • NEC 1 antibody
    • NEC1 antibody
    • NEC1_HUMAN antibody
    • Neuroendocrine convertase 1 antibody
    • PC1 antibody
    • PC3 antibody
    • PCSK1 antibody
    • Prohormone convertase 1 antibody
    • Prohormone convertase 1/3 antibody
    • Prohormone convertase 3 antibody
    • Proprotein convertase 1 antibody
    • SPC3 antibody
    see all

图片

  • Western blot - Anti-PC1/3 antibody (ab3532)
    Western blot - Anti-PC1/3 antibody (ab3532)
    Western blot detection of PC1/3 from mouse pituitary extract using ab3532.
  • Western blot - Anti-PC1/3 antibody (ab3532)
    Western blot - Anti-PC1/3 antibody (ab3532)
    Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 68-87 kDa why is the actual band size different from the predicted?
    Additional bands at: 35 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.

文献 (4)

发表研究结果有使用 ab3532？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3532 被引用在 4 文献中.

  • Saikia M  et al. GLP-1 receptor signaling increases PCSK1 and ß cell features in human a cells. JCI Insight 6:N/A (2021). PubMed: 33554958
  • Razolli DS  et al. Proopiomelanocortin Processing in the Hypothalamus Is Directly Regulated by Saturated Fat: Implications for the Development of Obesity. Neuroendocrinology 110:92-104 (2020). PubMed: 31104058
  • Irwin N  et al. Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice. Biochem Pharmacol : (2012). PubMed: 23085436
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-PC1/3 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse brain (hypothalamus))
Permeabilization
No
Specification
mouse brain (hypothalamus)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ali Seifinejad

Verified customer

提交于 May 02 2017

Question

A replacement vial for ab3532 would be great. I do believe it was the delay in shipping that is the source of the problem.

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Answer

I have received the following information from the scientist who tested ab3532: "The alignment of the C-terminal peptides shows non cross reactivity to mouse is not surprising since there are not 5 contiguous amino acids that are identical in both sequences of mouse and human". I have updated this information on the datasheet and believe therefore that a replacement vial will not give you better results than the original vial. Would you like to have a replacement vial of another antibody? I look forward to hearing from you to arrange a new free order,

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Question

BATCH NUMBER 90293 ORDER NUMBER 147797 DESCRIPTION OF THE PROBLEM no bands SAMPLE STC-1 cells (mouse intestinal endocrine) lysates PRIMARY ANTIBODY abcam rabbit anti-PC1(ab12275) or abcam rabbit anti-PC2(ab3532), 1:2500 or 1:500, 0.01%tween-20/PBS, 1hr RT or overnight 4C plus 1hr RT washing: 2x 1min, 1x 15min, 3x 5 min DETECTION METHOD ECL Plus (Amersham, cat RPN2132) POSITIVE AND NEGATIVE CONTROLS USED loaded STC-1 lysates ANTIBODY STORAGE CONDITIONS antibody dispensed in 2uL aliquots and stored at -20C SAMPLE PREPARATION lysate buffer: complete mini protease inhibitor (Roche, cat #11836153001) AMOUNT OF PROTEIN LOADED tried 10 and 20ug of total protein lysates per lane ELECTROPHORESIS/GEL CONDITIONS 95C at 5 min, DTT, NuPAGE 4-12% Bis-Tris gel (Invitrogen), 150V 1.5 hrs TRANSFER AND BLOCKING CONDITIONS 10% methanol/NuPAGE transfer buffer, 30 V 1.5 hrs, blocking: 5% skim milk/PBS/ 0.01% tween-20 SECONDARY ANTIBODY goat anti-rabbit HRP conjugated (another company), 1:10,000, diluted in 0.01% tween-20/PBS washing: 2x 1min, 1x 15min, 3x 5 min HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? 1) increased primary antibody conc from 1:2500 to 1:500 2) increased incubation time from 1hr RT to overnight at 4c plus 1hr RT 3) increased secondary antibody conc from 1:10,000 to 1:5,000 ADDITIONAL NOTES To test whether any protein was transferred, I probed the same membrane that did not yield any bands with the anti-PC1/PC2 antibodies, with anti-B tubulin and then HRP conjugated secondary. Using this antibody I was able to detect the appropriate bands for this ubiquitous protein.

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Answer

Thank you very much for your protocol details and your order number. I firstly looked at your protocol and think it is very good. I found references for the presence of both PC1 and PC2 in STC-1 cells (Yoon et al, Endocrinol. 1997 for example) and therefore the problem is certainly not due to low amounts of the protein in your samples. However, we have not determined whether ab12275 does cross react with mouse PC2 and I am currently enquiring to find out the exact homology of the immunogen with the mouse protein; I performed a homology search with the last 15 amino acids which contain the immunogen and found 86% homology. I think therefore that it would be important to determine the exact homology so that we can determine if the problem with ab12275 is a species cross reactivity problem. I secondly looked at the shipping records of your order and indeed found a delay; the package was held by regulatory agency on May 24th and I am worried that the package was stored in a hot area. I would like to offer you a replacement vial of ab3532 already and to wait for the cross reactivity information regarding ab12275, as if it is unlikely to cross react with mouse I would recommend to use a different antibody to detect PC2. I appreciate your patience and will e-mail you as soon as I find out the missing information regarding ab12275,

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