Anti-PBK/SPK抗体(ab226923)
Key features and details
- Rabbit polyclonal to PBK/SPK
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PBK/SPK抗体
参阅全部 PBK/SPK 一抗 -
描述
兔多克隆抗体to PBK/SPK -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Chimpanzee
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免疫原
Recombinant fragment within Human PBK/SPK (internal sequence). The exact sequence is proprietary.
Database link: Q96KB5 -
阳性对照
- WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IP: A431 whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 36,37 kDa.
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| 说明 |
|---|
|
IP
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 36,37 kDa. |
靶标
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功能
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. -
组织特异性
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated; in a cell-cycle dependent manner at mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 464496 Chimpanzee
- Entrez Gene: 534781 Cow
- Entrez Gene: 55872 Human
- Entrez Gene: 52033 Mouse
- Entrez Gene: 100141310 Pig
- Entrez Gene: 290326 Rat
- Omim: 611210 Human
- SwissProt: Q96KB5 Human
see all -
别名
- Cancer/testis antigen 84 antibody
- CT84 antibody
- Epididymis luminal protein 164 antibody
see all
图片
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Western blot - Anti-PBK/SPK antibody (ab226923)All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-PBK/SPK antibody (ab226923)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PBK/SPK (green) using ab226923 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).
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Immunoprecipitation - Anti-PBK/SPK antibody (ab226923)PBK/SPK was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226923. Western blot was performed from the immunoprecipitate using ab226923. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: A431 whole cell extract (Input).
Lane 2: Control IgG IP in A431 whole cell extract.
Lane 3: ab226923 IP in A431 whole cell extract.
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Western blot - Anti-PBK/SPK antibody (ab226923)All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab226923 被引用在 2 文献中.
- Jung M et al. Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients. Cancer Res Commun 2:1617-1625 (2022). PubMed: 36970725
- Mao P et al. PDZ-Binding Kinase-Dependent Transcriptional Regulation of CCNB2 Promotes Tumorigenesis and Radio-Resistance in Glioblastoma. Transl Oncol 13:287-294 (2020). PubMed: 31874375