AB109547

重组Anti-Paxillin (phospho Y118)抗体[EPR1903]

Anti-Paxillin (phospho Y118) antibody [EPR1903]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Paxillin phospho Y118 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.

查看别名

Paxillin, PXN

3 Images

Unknown

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

Jurkat treated with 50mM pervanadate for 5 min whole cell lysate at 10 µg

Lane 3:

Jurkat treated with 50mM pervanadate for 5 min whole cell lysate, then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

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Unknown

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

All lanes:

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with pervanadate at 10 µg

Predicted band size: 46 kDa,65 kDa

false

Unknown

Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20000. Blocking buffer : 5% NFDM/BST. Dilution buffer : 5% NFDM /TBST.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1903

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Dot, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.

Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.

See full target information Paxillin phospho Y118

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:46 PubMed38233791

2024

ZDHHC5-mediated S-palmitoylation of FAK promotes its membrane localization and epithelial-mesenchymal transition in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Na Shen,Yang Yang,Yuan Xia,Wenhao Zhang,Yu Lu,Zhicheng Wang,Ze Yang,Zhangjie Wang

Bioactive materials 20:81-92 PubMed35633875

2022

Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochan Li,Boon Chin Heng,Yunyang Bai,Qianqian Wang,Min Gao,Ying He,Xinwen Zhang,Xuliang Deng,Xuehui Zhang

Frontiers in pharmacology 13:855626 PubMed35656311

2022

Resibufogenin Targets the ATP1A1 Signaling Cascade to Induce G2/M Phase Arrest and Inhibit Invasion in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhang,Zhong Yao,Zhiyi Xue,Shuai Wang,Xuemeng Liu,Yaotian Hu,Yan Zhang,Jian Wang,Xingang Li,Anjing Chen

Phytomedicine : international journal of phytotherapy and phytopharmacology 96:153908 PubMed35026516

2022

Astragaloside IV promotes the angiogenic capacity of adipose-derived mesenchymal stem cells in a hindlimb ischemia model by FAK phosphorylation via CXCR2.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyi Wang,Zekun Shen,Yanan Tang,Bingyi Chen,Jinxing Chen,Jiaxuan Hou,Jiayan Li,Mengzhao Zhang,Shuang Liu,Yifan Mei,Liwei Zhang,Shaoying Lu

Biochemical pharmacology 195:114864 PubMed34861243

2021

Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Yu,Lijing Zhang,Jun Peng,Richard Ward,Peiqi Hao,Jiwei Wang,Na Zhang,Yang Yang,Xiaoxi Guo,Cheng Xiang,Su An,Tian-Rui Xu

Journal of clinical laboratory analysis 35:e24044 PubMed34714955

2021

miR-423-3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ma,Wuhao Huang,Chaonan Zhu,Xiaoyan Sun,Qiang Zhang,Lianmin Zhang,Qi Qi,Xiaoming Bai,Yun Feng,Changli Wang

Molecular therapy. Nucleic acids 25:567-577 PubMed34589278

2021

miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Zhang,Liwen Yao,Wei Zhou,Jinping Tian,Banlai Ruan,Zihua Lu,Yunchao Deng,Qing Li,Zhi Zeng,Dongmei Yang,Renduo Shang,Ming Xu,Mengjiao Zhang,Du Cheng,Yanning Yang,Qianshan Ding,Honggang Yu

Nature communications 9:5105 PubMed30504771

2018

Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Chen Liu,Tien Hsu,Yu-Sun Chang,An-Ko Chung,Shih Sheng Jiang,Chun-Nan OuYang,Chiou-Hwa Yuh,Chuen Hsueh,Ya-Ping Liu,Ngan-Ming Tsang

Oncotarget 7:72113-72130 PubMed27708220

2016

Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity.

Applications