Anti-Paxillin (phospho Y118)抗体(ab75740)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y118)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Paxillin (phospho Y118)抗体
参阅全部 Paxillin 一抗 -
描述
兔多克隆抗体to Paxillin (phospho Y118) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Paxillin (phospho Y118).
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阳性对照
- Human breast carcinoma tissue. Extracts from HeLa cells treated with EGF.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75740于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
序列相似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻译后修饰
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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数据库链接
- Entrez Gene: 5829 Human
- Omim: 602505 Human
- SwissProt: P49023 Human
- Unigene: 446336 Human
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别名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin (phospho Y118) antibody (ab75740)ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-Paxillin (phospho Y118) antibody (ab75740)All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution
Lane 1 : HeLa cell extract, untreated
Lane 2 : HeLa cell extract treated with EGF
Predicted band size: 64 kDa
Observed band size: 64 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab75740 被引用在 4 文献中.
- Qiu W et al. Hypoxia-induced EPHB2 promotes invasive potential of glioblastoma. Int J Clin Exp Pathol 12:539-548 (2019). PubMed: 31933858
- Cheng Y et al. Depression promotes prostate cancer invasion and metastasis via a sympathetic-cAMP-FAK signaling pathway. Oncogene 37:2953-2966 (2018). PubMed: 29515233
- Alsaffar H et al. Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis. Am J Physiol Cell Physiol 314:C589-C602 (2018). PubMed: 29351406
- Adam AP et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016). PubMed: 27603666