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Anti-Paxillin (phospho S83)抗体(ab31833)

Submit a review Q&A (1)
Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)

    Key features and details

    • Rabbit polyclonal to Paxillin (phospho S83)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Paxillin (phospho S83)抗体
      参阅全部 Paxillin 一抗

    • 描述

      兔多克隆抗体to Paxillin (phospho S83)

    • 宿主

      Rabbit

    • 特异性

      ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Synthetic peptide corresponding to Mouse Paxillin (phospho S83) conjugated to keyhole limpet haemocyanin.
      Database link: Q8VI36

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab31833于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Predicted molecular weight: 68 kDa.

    In 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
    说明
    WB
    1/1000. Predicted molecular weight: 68 kDa.

    In 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

    靶标

    • 功能

      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

    • 序列相似性

      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.

    • 翻译后修饰

      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.

    • 细胞定位

      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt

    • 数据库链接

      • 别名

        • FLJ16691 antibody
        • FLJ23042 antibody
        • Paired box protein Pax 1 antibody
        • PAX 1 antibody
        • PAX1 antibody
        • PAXI_HUMAN antibody
        • Paxillin alpha antibody
        • Paxillin antibody
        • PXN antibody
        • PXN protein antibody
        see all

      图片

      • Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)
        Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)
        Western blot analysis of immunoprecipitated paxillin (arrow) in mouse macrophages untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min (lanes 2, 4, 5, & 6). Lysates were immunoprecipitated with anti-Paxillin. The blots were probed with anti-Paxillin or anti-phospho-Paxillin (Ser-83). The latter was used in the presence of phospho-Paxillin (Ser-83) peptide (lane 5) or phospho-Paxillin (Ser-85) peptide (lane 6). Immunoglobulin (Ig) chains from the immunoprecipitation were observed above and below the paxillin bands.

      文献 (0)

      发表研究结果有使用 ab31833？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab31833 尚未被引用在任何文献中。

      客户评价及客户问答

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      提交评价 提交问题

      Question

      Does the Paxillin (phospho S83) antibody (ab31833) react against human or monkey lysates? I would like to test for phosphorylation of the equivalent residue in cell lines of these organisms and can't find a human or monkey specific antibody. As such, I would be willing to test whether or not this antibody would react with phosphorylated money or human paxillin. Do you cell the positive control lysates (mouse macrophages treated with Calyculin A?) Your help would be appreciated.

      Read More
      Answer

      Thank you for your enquiry. This antibody has yet to be tested in human or monkey tissue. The conserved site in human paxillin (Ser-83) has high homology with the mouse paxillin (Ser-83). There is only 2 amino acid differences, however there is evidence that in human the phosphorylation site is Ser-85 rather than Ser-83 (Journal of Cell Biology, (2004) Vol 164(4):593-602.). We have only shown that ab31833 works in mouse, but it may be useful for detecting Ser-83 in human. We currently do not sell the positive control I'm afraid. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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