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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions

Anti-Paxillin抗体(ab2264)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (14)

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Western blot - Anti-Paxillin antibody (ab2264)

    Key features and details

    • Rabbit polyclonal to Paxillin
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Paxillin antibody [E228] (ab32115)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Paxillin抗体
      参阅全部 Paxillin 一抗
    • 描述

      兔多克隆抗体to Paxillin
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse
      预测可用于: Rat, Chicken, Human
    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Focal Adhesions
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Cancer
      • Signal transduction
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • BALB/3T3 whole cell lysate (ab7901)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2264于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 68 kDa.
    说明
    WB
    1/500 - 1/1000. Predicted molecular weight: 68 kDa.

    靶标

    • 功能

      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • 序列相似性

      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • 翻译后修饰

      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • 细胞定位

      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Target information above from: UniProt accession P49023 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 395832 Chicken
      • Entrez Gene: 5829 Human
      • Entrez Gene: 19303 Mouse
      • Entrez Gene: 360820 Rat
      • Omim: 602505 Human
      • SwissProt: P49024 Chicken
      • SwissProt: P49023 Human
      • SwissProt: Q8VI36 Mouse
      • SwissProt: Q66H76 Rat
      • Unigene: 446336 Human
      • Unigene: 18714 Mouse
      • Unigene: 75 Rat
      see all
    • 别名

      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    图片

    • Western blot - Anti-Paxillin antibody (ab2264)
      Western blot - Anti-Paxillin antibody (ab2264)
      Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 68 kDa
      Observed band size: 68 kDa
      Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      ab2264 detects a band at 68 kDa in NIH 3T3 lysate, as well as an additional band at 43kDa. An NCBI BLAST search of the peptide sequence identified an N terminal fragment of the Paxillin molecule with a primary molecular weight of 36 kDa. We believe the 43 kDa band observed may correspond to this fragment.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab2264?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2264 被引用在 14 文献中.

    • Wen L  et al. Paxillin knockdown suppresses metastasis and epithelial-mesenchymal transition in colorectal cancer via the ERK signalling pathway. Oncol Rep 44:1105-1115 (2020). PubMed: 32705241
    • Ghate K  et al. Fmn2 Regulates Growth Cone Motility by Mediating a Molecular Clutch to Generate Traction Forces. Neuroscience 448:160-171 (2020). PubMed: 33002558
    • Neganova I  et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). PubMed: 30512203
    • Ghosh M  et al. CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, ß1 integrin recycling, and cell migration. Sci Signal 12:N/A (2019). PubMed: 31040262
    • Luan L  et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). PubMed: 30186380
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Paxillin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MDA-MB-435S)
    Specification
    MDA-MB-435S
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 28 2013

    Immunoprecipitation abreview for Anti-Paxillin antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS)
    Total protein in input
    400 µg
    Specification
    U2OS
    Immuno-precipitation step
    Protein A
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 28 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Paxillin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human (U2OS) mouse (MEF) rat (RIE1))
    Specification
    Human (U2OS) mouse (MEF) rat (RIE1)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Triton-100 [PBS]
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 10 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Paxillin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human and mouse (pancreas), rat (testis))
    Specification
    human and mouse (pancreas), rat (testis)
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    No
    Blocking step
    Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 08 2008

    Western blot abreview for Anti-Paxillin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS, NIH-3T3, Raw264.7, PC12)
    Loading amount
    20 µg
    Specification
    U2OS, NIH-3T3, Raw264.7, PC12
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 04 2008

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